<i>In Vitro</i> Culture of Turkish <i>Origanum sipyleum</i> L.

Başar Sevindik; Tolga İzgü; Özhan Şimşek; Mehmet Tütüncü; Pembe Çürük; Özer Yılmaz; Gönül Kaynak; Yıldız Aka Kaçar; Jaime A. Teixeira da Silva; Yeşim Yalçın Mendi

doi:doi:10.11648/j.ajpb.s.2017020301.16

In Vitro Culture of Turkish Origanum sipyleum L.

Başar Sevindik, Tolga İzgü, Özhan Şimşek, Mehmet Tütüncü, Pembe Çürük, Özer Yılmaz, Gönül Kaynak, Yıldız Aka Kaçar, Jaime A. Teixeira da Silva, Yeşim Yalçın Mendi

Published in American Journal of Plant Biology (Volume 2, Issue 3-1)

Received: 20 April 2017 Accepted: 21 April 2017 Published: 17 July 2017

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Abstract

Origanum sipyleum L. is a species that is endemic to Turkey with known medicinal and aromatic properties and with a long historical use in Turkish folk medicine. Micropropagation serves as one possible method to clone and protect O. sipyleum. In this study, nodes, which served as the explants, were cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of BA (0.1, 0.5, 1.0 mgL^-1) and GA₃ (0.1, 0.2 mgL^-1). Shoots were transferred to MS medium containing different concentrations of IBA (0, 0.5, 1, 1.5, 2, 2.5, 3 mgL^-1) for root induction. Shoot regeneration, rooting, survival and acclimatization were possible. As many as 85% of nodes formed an average of 6 shoots/node on MS medium supplemented with 0.5 mgL^-1 BA and 0.2 mgL^-1 GA₃. Rooting was most successful in 62.5% of shoots on MS medium with 1.5 or 2.5 mgL^-1 IBA. Plantlets derived from all IBA combinations were transferred to autoclaved peat and perlite (1:1, v/v) and acclimatized in a greenhouse, with 79.1% survival. This protocol represents the first comprehensive in vitro regeneration protocol for endemic (Turkish) O. sipyleum.

Published in	American Journal of Plant Biology (Volume 2, Issue 3-1) This article belongs to the Special Issue Plant Molecular Biology and Biotechnology
DOI	10.11648/j.ajpb.s.2017020301.16
Page(s)	32-36
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

BA, GA₃, IBA, MS Medium, Tissue Culture

References

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APA Style

Başar Sevindik, Tolga İzgü, Özhan Şimşek, Mehmet Tütüncü, Pembe Çürük, et al. (2017). In Vitro Culture of Turkish Origanum sipyleum L.. American Journal of Plant Biology, 2(3-1), 32-36. https://doi.org/10.11648/j.ajpb.s.2017020301.16

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ACS Style

Başar Sevindik; Tolga İzgü; Özhan Şimşek; Mehmet Tütüncü; Pembe Çürük, et al. In Vitro Culture of Turkish Origanum sipyleum L.. Am. J. Plant Biol. 2017, 2(3-1), 32-36. doi: 10.11648/j.ajpb.s.2017020301.16

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AMA Style

Başar Sevindik, Tolga İzgü, Özhan Şimşek, Mehmet Tütüncü, Pembe Çürük, et al. In Vitro Culture of Turkish Origanum sipyleum L.. Am J Plant Biol. 2017;2(3-1):32-36. doi: 10.11648/j.ajpb.s.2017020301.16

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@article{10.11648/j.ajpb.s.2017020301.16,
  author = {Başar Sevindik and Tolga İzgü and Özhan Şimşek and Mehmet Tütüncü and Pembe Çürük and Özer Yılmaz and Gönül Kaynak and Yıldız Aka Kaçar and Jaime A. Teixeira da Silva and Yeşim Yalçın Mendi},
  title = {In Vitro Culture of Turkish Origanum sipyleum L.},
  journal = {American Journal of Plant Biology},
  volume = {2},
  number = {3-1},
  pages = {32-36},
  doi = {10.11648/j.ajpb.s.2017020301.16},
  url = {https://doi.org/10.11648/j.ajpb.s.2017020301.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.s.2017020301.16},
  abstract = {Origanum sipyleum L. is a species that is endemic to Turkey with known medicinal and aromatic properties and with a long historical use in Turkish folk medicine. Micropropagation serves as one possible method to clone and protect O. sipyleum. In this study, nodes, which served as the explants, were cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of BA (0.1, 0.5, 1.0 mgL-1) and GA3 (0.1, 0.2 mgL-1). Shoots were transferred to MS medium containing different concentrations of IBA (0, 0.5, 1, 1.5, 2, 2.5, 3 mgL-1) for root induction. Shoot regeneration, rooting, survival and acclimatization were possible. As many as 85% of nodes formed an average of 6 shoots/node on MS medium supplemented with 0.5 mgL-1 BA and 0.2 mgL-1 GA3. Rooting was most successful in 62.5% of shoots on MS medium with 1.5 or 2.5 mgL-1 IBA. Plantlets derived from all IBA combinations were transferred to autoclaved peat and perlite (1:1, v/v) and acclimatized in a greenhouse, with 79.1% survival. This protocol represents the first comprehensive in vitro regeneration protocol for endemic (Turkish) O. sipyleum.},
 year = {2017}
}

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TY  - JOUR
T1  - In Vitro Culture of Turkish Origanum sipyleum L.
AU  - Başar Sevindik
AU  - Tolga İzgü
AU  - Özhan Şimşek
AU  - Mehmet Tütüncü
AU  - Pembe Çürük
AU  - Özer Yılmaz
AU  - Gönül Kaynak
AU  - Yıldız Aka Kaçar
AU  - Jaime A. Teixeira da Silva
AU  - Yeşim Yalçın Mendi
Y1  - 2017/07/17
PY  - 2017
N1  - https://doi.org/10.11648/j.ajpb.s.2017020301.16
DO  - 10.11648/j.ajpb.s.2017020301.16
T2  - American Journal of Plant Biology
JF  - American Journal of Plant Biology
JO  - American Journal of Plant Biology
SP  - 32
EP  - 36
PB  - Science Publishing Group
SN  - 2578-8337
UR  - https://doi.org/10.11648/j.ajpb.s.2017020301.16
AB  - Origanum sipyleum L. is a species that is endemic to Turkey with known medicinal and aromatic properties and with a long historical use in Turkish folk medicine. Micropropagation serves as one possible method to clone and protect O. sipyleum. In this study, nodes, which served as the explants, were cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of BA (0.1, 0.5, 1.0 mgL-1) and GA3 (0.1, 0.2 mgL-1). Shoots were transferred to MS medium containing different concentrations of IBA (0, 0.5, 1, 1.5, 2, 2.5, 3 mgL-1) for root induction. Shoot regeneration, rooting, survival and acclimatization were possible. As many as 85% of nodes formed an average of 6 shoots/node on MS medium supplemented with 0.5 mgL-1 BA and 0.2 mgL-1 GA3. Rooting was most successful in 62.5% of shoots on MS medium with 1.5 or 2.5 mgL-1 IBA. Plantlets derived from all IBA combinations were transferred to autoclaved peat and perlite (1:1, v/v) and acclimatized in a greenhouse, with 79.1% survival. This protocol represents the first comprehensive in vitro regeneration protocol for endemic (Turkish) O. sipyleum.
VL  - 2
IS  - 3-1
ER  -

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Author Information

Başar Sevindik
Tolga İzgü
Özhan Şimşek
Mehmet Tütüncü

Department of Horticulture, Faculty of Agriculture, University of Ondokuz May?s Atakum, Samsun, Turkey
Pembe Çürük
Özer Yılmaz
Gönül Kaynak
Yıldız Aka Kaçar
Jaime A. Teixeira da Silva

Miki-cho Post Office, Ikenobe, Kagawa-ken, Japan
Yeşim Yalçın Mendi

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APA Style

Başar Sevindik, Tolga İzgü, Özhan Şimşek, Mehmet Tütüncü, Pembe Çürük, et al. (2017). In Vitro Culture of Turkish Origanum sipyleum L.. American Journal of Plant Biology, 2(3-1), 32-36. https://doi.org/10.11648/j.ajpb.s.2017020301.16

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ACS Style

Başar Sevindik; Tolga İzgü; Özhan Şimşek; Mehmet Tütüncü; Pembe Çürük, et al. In Vitro Culture of Turkish Origanum sipyleum L.. Am. J. Plant Biol. 2017, 2(3-1), 32-36. doi: 10.11648/j.ajpb.s.2017020301.16

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AMA Style

Başar Sevindik, Tolga İzgü, Özhan Şimşek, Mehmet Tütüncü, Pembe Çürük, et al. In Vitro Culture of Turkish Origanum sipyleum L.. Am J Plant Biol. 2017;2(3-1):32-36. doi: 10.11648/j.ajpb.s.2017020301.16

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@article{10.11648/j.ajpb.s.2017020301.16,
  author = {Başar Sevindik and Tolga İzgü and Özhan Şimşek and Mehmet Tütüncü and Pembe Çürük and Özer Yılmaz and Gönül Kaynak and Yıldız Aka Kaçar and Jaime A. Teixeira da Silva and Yeşim Yalçın Mendi},
  title = {In Vitro Culture of Turkish Origanum sipyleum L.},
  journal = {American Journal of Plant Biology},
  volume = {2},
  number = {3-1},
  pages = {32-36},
  doi = {10.11648/j.ajpb.s.2017020301.16},
  url = {https://doi.org/10.11648/j.ajpb.s.2017020301.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.s.2017020301.16},
  abstract = {Origanum sipyleum L. is a species that is endemic to Turkey with known medicinal and aromatic properties and with a long historical use in Turkish folk medicine. Micropropagation serves as one possible method to clone and protect O. sipyleum. In this study, nodes, which served as the explants, were cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of BA (0.1, 0.5, 1.0 mgL-1) and GA3 (0.1, 0.2 mgL-1). Shoots were transferred to MS medium containing different concentrations of IBA (0, 0.5, 1, 1.5, 2, 2.5, 3 mgL-1) for root induction. Shoot regeneration, rooting, survival and acclimatization were possible. As many as 85% of nodes formed an average of 6 shoots/node on MS medium supplemented with 0.5 mgL-1 BA and 0.2 mgL-1 GA3. Rooting was most successful in 62.5% of shoots on MS medium with 1.5 or 2.5 mgL-1 IBA. Plantlets derived from all IBA combinations were transferred to autoclaved peat and perlite (1:1, v/v) and acclimatized in a greenhouse, with 79.1% survival. This protocol represents the first comprehensive in vitro regeneration protocol for endemic (Turkish) O. sipyleum.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - In Vitro Culture of Turkish Origanum sipyleum L.
AU  - Başar Sevindik
AU  - Tolga İzgü
AU  - Özhan Şimşek
AU  - Mehmet Tütüncü
AU  - Pembe Çürük
AU  - Özer Yılmaz
AU  - Gönül Kaynak
AU  - Yıldız Aka Kaçar
AU  - Jaime A. Teixeira da Silva
AU  - Yeşim Yalçın Mendi
Y1  - 2017/07/17
PY  - 2017
N1  - https://doi.org/10.11648/j.ajpb.s.2017020301.16
DO  - 10.11648/j.ajpb.s.2017020301.16
T2  - American Journal of Plant Biology
JF  - American Journal of Plant Biology
JO  - American Journal of Plant Biology
SP  - 32
EP  - 36
PB  - Science Publishing Group
SN  - 2578-8337
UR  - https://doi.org/10.11648/j.ajpb.s.2017020301.16
AB  - Origanum sipyleum L. is a species that is endemic to Turkey with known medicinal and aromatic properties and with a long historical use in Turkish folk medicine. Micropropagation serves as one possible method to clone and protect O. sipyleum. In this study, nodes, which served as the explants, were cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of BA (0.1, 0.5, 1.0 mgL-1) and GA3 (0.1, 0.2 mgL-1). Shoots were transferred to MS medium containing different concentrations of IBA (0, 0.5, 1, 1.5, 2, 2.5, 3 mgL-1) for root induction. Shoot regeneration, rooting, survival and acclimatization were possible. As many as 85% of nodes formed an average of 6 shoots/node on MS medium supplemented with 0.5 mgL-1 BA and 0.2 mgL-1 GA3. Rooting was most successful in 62.5% of shoots on MS medium with 1.5 or 2.5 mgL-1 IBA. Plantlets derived from all IBA combinations were transferred to autoclaved peat and perlite (1:1, v/v) and acclimatized in a greenhouse, with 79.1% survival. This protocol represents the first comprehensive in vitro regeneration protocol for endemic (Turkish) O. sipyleum.
VL  - 2
IS  - 3-1
ER  -

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