Molecular expression analysis of different inflammatory mediators and their role in breast cancer progression and metastasis

Aula Ammar; Amani Halabi; Zuheir Al-Shehabi

doi:doi:10.11648/j.ajbls.s.2015030203.13

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Molecular expression analysis of different inflammatory mediators and their role in breast cancer progression and metastasis

Aula Ammar, Amani Halabi, Zuheir Al-Shehabi

Published in American Journal of Biomedical and Life Sciences (Volume 3, Issue 2-3)

Received: 7 December 2014 Accepted: 9 December 2014 Published: 7 August 2015

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Abstract

Introduction: Cytokines include different subfamilies such as interleukins (IL), chemokines, and growth factors. They play an important role in inflammatory conditions such as cancer progression and metastasis. There is an increasing interest in developing strategies to antagonize the function of some cytokine/chemokine to interfere with tumor progression and metastasis, the leading cause of death in most patients. The aim of the research project is to study the molecular characteristics of a sample of Syrian patients with breast cancer and assess the protein and gene expression of different inflammatory mediators and correlate that with the clinicopathological criteria of tumors. Materials and methods: Patient samples will be evaluated histologically (H&E stain) and stained immunohistochemically with antibodies against important molecular markers, cytokines, and different types of activated leukocytes. Immunohistochemistry of CD206, a marker of alternatively activated macrophages in tumors is shown here. PCR and immunohistochemical analysis of cytokines (e.g. IL-2, GM-CSF, IFNγ, M-CSG, IL-4, IL-10, CXCL8, CXCL12, CCL21, CCL19, CCR7) will be further implemented. The staining intensity, localization and distribution within the tumor will be examined and correlated with the gene expression and other clinnicopathological information. Expected results: We expect to get new information about the role of different cytokines in breast cancer progression in addition to get an insight into the possible inter-relationship between these cytokines.

Published in	American Journal of Biomedical and Life Sciences (Volume 3, Issue 2-3) This article belongs to the Special Issue Spectral Imaging for Medical Diagnosis “Modern Tool for Molecular Imaging”
DOI	10.11648/j.ajbls.s.2015030203.13
Page(s)	16-20
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Breast Cancer, Tumor Environment, Lymph Node Metastasis, Cytokines, Chemokines, Insert

References

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APA Style

Aula Ammar, Amani Halabi, Zuheir Al-Shehabi. (2015). Molecular expression analysis of different inflammatory mediators and their role in breast cancer progression and metastasis. American Journal of Biomedical and Life Sciences, 3(2-3), 16-20. https://doi.org/10.11648/j.ajbls.s.2015030203.13

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ACS Style

Aula Ammar; Amani Halabi; Zuheir Al-Shehabi. Molecular expression analysis of different inflammatory mediators and their role in breast cancer progression and metastasis. Am. J. Biomed. Life Sci. 2015, 3(2-3), 16-20. doi: 10.11648/j.ajbls.s.2015030203.13

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AMA Style

Aula Ammar, Amani Halabi, Zuheir Al-Shehabi. Molecular expression analysis of different inflammatory mediators and their role in breast cancer progression and metastasis. Am J Biomed Life Sci. 2015;3(2-3):16-20. doi: 10.11648/j.ajbls.s.2015030203.13

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@article{10.11648/j.ajbls.s.2015030203.13,
  author = {Aula Ammar and Amani Halabi and Zuheir Al-Shehabi},
  title = {Molecular expression analysis of different inflammatory mediators and their role in breast cancer progression and metastasis},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {3},
  number = {2-3},
  pages = {16-20},
  doi = {10.11648/j.ajbls.s.2015030203.13},
  url = {https://doi.org/10.11648/j.ajbls.s.2015030203.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.s.2015030203.13},
  abstract = {Introduction: Cytokines include different subfamilies such as interleukins (IL), chemokines, and growth factors. They play an important role in inflammatory conditions such as cancer progression and metastasis. There is an increasing interest in developing strategies to antagonize the function of some cytokine/chemokine to interfere with tumor progression and metastasis, the leading cause of death in most patients. The aim of the research project is to study the molecular characteristics of a sample of Syrian patients with breast cancer and assess the protein and gene expression of different inflammatory mediators and correlate that with the clinicopathological criteria of tumors. Materials and methods: Patient samples will be evaluated histologically (H&E stain) and stained immunohistochemically with antibodies against important molecular markers, cytokines, and different types of activated leukocytes. Immunohistochemistry of CD206, a marker of alternatively activated macrophages in tumors is shown here. PCR and immunohistochemical analysis of cytokines (e.g. IL-2, GM-CSF, IFNγ, M-CSG, IL-4, IL-10, CXCL8, CXCL12, CCL21, CCL19, CCR7) will be further implemented. The staining intensity, localization and distribution within the tumor will be examined and correlated with the gene expression and other clinnicopathological information. Expected results: We expect to get new information about the role of different cytokines in breast cancer progression in addition to get an insight into the possible inter-relationship between these cytokines.},
 year = {2015}
}

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TY  - JOUR
T1  - Molecular expression analysis of different inflammatory mediators and their role in breast cancer progression and metastasis
AU  - Aula Ammar
AU  - Amani Halabi
AU  - Zuheir Al-Shehabi
Y1  - 2015/08/07
PY  - 2015
N1  - https://doi.org/10.11648/j.ajbls.s.2015030203.13
DO  - 10.11648/j.ajbls.s.2015030203.13
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 16
EP  - 20
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.s.2015030203.13
AB  - Introduction: Cytokines include different subfamilies such as interleukins (IL), chemokines, and growth factors. They play an important role in inflammatory conditions such as cancer progression and metastasis. There is an increasing interest in developing strategies to antagonize the function of some cytokine/chemokine to interfere with tumor progression and metastasis, the leading cause of death in most patients. The aim of the research project is to study the molecular characteristics of a sample of Syrian patients with breast cancer and assess the protein and gene expression of different inflammatory mediators and correlate that with the clinicopathological criteria of tumors. Materials and methods: Patient samples will be evaluated histologically (H&E stain) and stained immunohistochemically with antibodies against important molecular markers, cytokines, and different types of activated leukocytes. Immunohistochemistry of CD206, a marker of alternatively activated macrophages in tumors is shown here. PCR and immunohistochemical analysis of cytokines (e.g. IL-2, GM-CSF, IFNγ, M-CSG, IL-4, IL-10, CXCL8, CXCL12, CCL21, CCL19, CCR7) will be further implemented. The staining intensity, localization and distribution within the tumor will be examined and correlated with the gene expression and other clinnicopathological information. Expected results: We expect to get new information about the role of different cytokines in breast cancer progression in addition to get an insight into the possible inter-relationship between these cytokines.
VL  - 3
IS  - 2-3
ER  -

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Author Information

Aula Ammar

Department of Biochemistry and Microbiology, School of Pharmacy, Tishreen University, Latakia, Syria
Amani Halabi

Department of Biochemistry and Microbiology, School of Pharmacy, Tishreen University, Latakia, Syria
Zuheir Al-Shehabi

Department of Pathology, School of Medicine, Tishreen University, Latakia, Syria

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APA Style

Aula Ammar, Amani Halabi, Zuheir Al-Shehabi. (2015). Molecular expression analysis of different inflammatory mediators and their role in breast cancer progression and metastasis. American Journal of Biomedical and Life Sciences, 3(2-3), 16-20. https://doi.org/10.11648/j.ajbls.s.2015030203.13

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ACS Style

Aula Ammar; Amani Halabi; Zuheir Al-Shehabi. Molecular expression analysis of different inflammatory mediators and their role in breast cancer progression and metastasis. Am. J. Biomed. Life Sci. 2015, 3(2-3), 16-20. doi: 10.11648/j.ajbls.s.2015030203.13

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AMA Style

Aula Ammar, Amani Halabi, Zuheir Al-Shehabi. Molecular expression analysis of different inflammatory mediators and their role in breast cancer progression and metastasis. Am J Biomed Life Sci. 2015;3(2-3):16-20. doi: 10.11648/j.ajbls.s.2015030203.13

Copy | Download

@article{10.11648/j.ajbls.s.2015030203.13,
  author = {Aula Ammar and Amani Halabi and Zuheir Al-Shehabi},
  title = {Molecular expression analysis of different inflammatory mediators and their role in breast cancer progression and metastasis},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {3},
  number = {2-3},
  pages = {16-20},
  doi = {10.11648/j.ajbls.s.2015030203.13},
  url = {https://doi.org/10.11648/j.ajbls.s.2015030203.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.s.2015030203.13},
  abstract = {Introduction: Cytokines include different subfamilies such as interleukins (IL), chemokines, and growth factors. They play an important role in inflammatory conditions such as cancer progression and metastasis. There is an increasing interest in developing strategies to antagonize the function of some cytokine/chemokine to interfere with tumor progression and metastasis, the leading cause of death in most patients. The aim of the research project is to study the molecular characteristics of a sample of Syrian patients with breast cancer and assess the protein and gene expression of different inflammatory mediators and correlate that with the clinicopathological criteria of tumors. Materials and methods: Patient samples will be evaluated histologically (H&E stain) and stained immunohistochemically with antibodies against important molecular markers, cytokines, and different types of activated leukocytes. Immunohistochemistry of CD206, a marker of alternatively activated macrophages in tumors is shown here. PCR and immunohistochemical analysis of cytokines (e.g. IL-2, GM-CSF, IFNγ, M-CSG, IL-4, IL-10, CXCL8, CXCL12, CCL21, CCL19, CCR7) will be further implemented. The staining intensity, localization and distribution within the tumor will be examined and correlated with the gene expression and other clinnicopathological information. Expected results: We expect to get new information about the role of different cytokines in breast cancer progression in addition to get an insight into the possible inter-relationship between these cytokines.},
 year = {2015}
}

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TY  - JOUR
T1  - Molecular expression analysis of different inflammatory mediators and their role in breast cancer progression and metastasis
AU  - Aula Ammar
AU  - Amani Halabi
AU  - Zuheir Al-Shehabi
Y1  - 2015/08/07
PY  - 2015
N1  - https://doi.org/10.11648/j.ajbls.s.2015030203.13
DO  - 10.11648/j.ajbls.s.2015030203.13
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 16
EP  - 20
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.s.2015030203.13
AB  - Introduction: Cytokines include different subfamilies such as interleukins (IL), chemokines, and growth factors. They play an important role in inflammatory conditions such as cancer progression and metastasis. There is an increasing interest in developing strategies to antagonize the function of some cytokine/chemokine to interfere with tumor progression and metastasis, the leading cause of death in most patients. The aim of the research project is to study the molecular characteristics of a sample of Syrian patients with breast cancer and assess the protein and gene expression of different inflammatory mediators and correlate that with the clinicopathological criteria of tumors. Materials and methods: Patient samples will be evaluated histologically (H&E stain) and stained immunohistochemically with antibodies against important molecular markers, cytokines, and different types of activated leukocytes. Immunohistochemistry of CD206, a marker of alternatively activated macrophages in tumors is shown here. PCR and immunohistochemical analysis of cytokines (e.g. IL-2, GM-CSF, IFNγ, M-CSG, IL-4, IL-10, CXCL8, CXCL12, CCL21, CCL19, CCR7) will be further implemented. The staining intensity, localization and distribution within the tumor will be examined and correlated with the gene expression and other clinnicopathological information. Expected results: We expect to get new information about the role of different cytokines in breast cancer progression in addition to get an insight into the possible inter-relationship between these cytokines.
VL  - 3
IS  - 2-3
ER  -

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