Grape Seed Proanthocyanidin Extract Down-Regulates Autophagic Proteins (LC3II and Beclin-1) in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury

Li Luo; Xing Tu; Wei Liu; Xuexia Zhang; Yilin Liu; Wenyan Zhao; Junhua Yang; Mengxia Wang; Jing Liu

doi:doi:10.11648/j.cmr.20190801.13

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Grape Seed Proanthocyanidin Extract Down-Regulates Autophagic Proteins (LC3II and Beclin-1) in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury

Li Luo, Xing Tu, Wei Liu, Xuexia Zhang, Yilin Liu, Wenyan Zhao, Junhua Yang, Mengxia Wang, Jing Liu

Published in Clinical Medicine Research (Volume 8, Issue 1)

Received: 31 January 2019 Accepted: 11 March 2019 Published: 25 March 2019

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Abstract

Neonatal hypoxic-ischemic encephalopathy (HIE) is a severe brain disease that often leads to death or irreversible sequelae. The aim of this study was to determine the effect of a naturally active drug (grape seed proanthocyanidin extract [GSPE]) on the expression of autophagic proteins in a mouse model of neonatal hypoxic-ischemic brain injury. In this study immunofluorescence staining and Western blotting were used to detect the expression of autophagy markers (LC3II and beclin1) in the brains of neonatal mice with hypoxic-ischemic brain injuries after GSPE administration. Our study showed that GSPE pre-treatment down-regulates LC3II and beclin-1 expression, thus GSPE may be a potential drug for the treatment of HIE.

Published in	Clinical Medicine Research (Volume 8, Issue 1)
DOI	10.11648/j.cmr.20190801.13
Page(s)	16-20
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Grape Seed Proanthocyanidins Extract, Hypoxic-Ischemic Brain Injury, Autophagy

References

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APA Style

Li Luo, Xing Tu, Wei Liu, Xuexia Zhang, Yilin Liu, et al. (2019). Grape Seed Proanthocyanidin Extract Down-Regulates Autophagic Proteins (LC3II and Beclin-1) in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury. Clinical Medicine Research, 8(1), 16-20. https://doi.org/10.11648/j.cmr.20190801.13

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ACS Style

Li Luo; Xing Tu; Wei Liu; Xuexia Zhang; Yilin Liu, et al. Grape Seed Proanthocyanidin Extract Down-Regulates Autophagic Proteins (LC3II and Beclin-1) in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury. Clin. Med. Res. 2019, 8(1), 16-20. doi: 10.11648/j.cmr.20190801.13

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AMA Style

Li Luo, Xing Tu, Wei Liu, Xuexia Zhang, Yilin Liu, et al. Grape Seed Proanthocyanidin Extract Down-Regulates Autophagic Proteins (LC3II and Beclin-1) in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury. Clin Med Res. 2019;8(1):16-20. doi: 10.11648/j.cmr.20190801.13

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@article{10.11648/j.cmr.20190801.13,
  author = {Li Luo and Xing Tu and Wei Liu and Xuexia Zhang and Yilin Liu and Wenyan Zhao and Junhua Yang and Mengxia Wang and Jing Liu},
  title = {Grape Seed Proanthocyanidin Extract Down-Regulates Autophagic Proteins (LC3II and Beclin-1) in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury},
  journal = {Clinical Medicine Research},
  volume = {8},
  number = {1},
  pages = {16-20},
  doi = {10.11648/j.cmr.20190801.13},
  url = {https://doi.org/10.11648/j.cmr.20190801.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cmr.20190801.13},
  abstract = {Neonatal hypoxic-ischemic encephalopathy (HIE) is a severe brain disease that often leads to death or irreversible sequelae. The aim of this study was to determine the effect of a naturally active drug (grape seed proanthocyanidin extract [GSPE]) on the expression of autophagic proteins in a mouse model of neonatal hypoxic-ischemic brain injury. In this study immunofluorescence staining and Western blotting were used to detect the expression of autophagy markers (LC3II and beclin1) in the brains of neonatal mice with hypoxic-ischemic brain injuries after GSPE administration. Our study showed that GSPE pre-treatment down-regulates LC3II and beclin-1 expression, thus GSPE may be a potential drug for the treatment of HIE.},
 year = {2019}
}

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TY  - JOUR
T1  - Grape Seed Proanthocyanidin Extract Down-Regulates Autophagic Proteins (LC3II and Beclin-1) in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury
AU  - Li Luo
AU  - Xing Tu
AU  - Wei Liu
AU  - Xuexia Zhang
AU  - Yilin Liu
AU  - Wenyan Zhao
AU  - Junhua Yang
AU  - Mengxia Wang
AU  - Jing Liu
Y1  - 2019/03/25
PY  - 2019
N1  - https://doi.org/10.11648/j.cmr.20190801.13
DO  - 10.11648/j.cmr.20190801.13
T2  - Clinical Medicine Research
JF  - Clinical Medicine Research
JO  - Clinical Medicine Research
SP  - 16
EP  - 20
PB  - Science Publishing Group
SN  - 2326-9057
UR  - https://doi.org/10.11648/j.cmr.20190801.13
AB  - Neonatal hypoxic-ischemic encephalopathy (HIE) is a severe brain disease that often leads to death or irreversible sequelae. The aim of this study was to determine the effect of a naturally active drug (grape seed proanthocyanidin extract [GSPE]) on the expression of autophagic proteins in a mouse model of neonatal hypoxic-ischemic brain injury. In this study immunofluorescence staining and Western blotting were used to detect the expression of autophagy markers (LC3II and beclin1) in the brains of neonatal mice with hypoxic-ischemic brain injuries after GSPE administration. Our study showed that GSPE pre-treatment down-regulates LC3II and beclin-1 expression, thus GSPE may be a potential drug for the treatment of HIE.
VL  - 8
IS  - 1
ER  -

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Author Information

Li Luo

School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Xing Tu

School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Wei Liu

School of Clinical Medicine, the First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Xuexia Zhang

Department of Anesthesiology, the Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, People's Republic of China
Yilin Liu

School of Clinical Medicine, the First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Wenyan Zhao

School of Clinical Medicine, the First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Junhua Yang

School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Mengxia Wang

Intensive Care Unit, Guangdong No. 2 Provincial People's Hospital, Guangzhou, People's Republic of China
Jing Liu

School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China

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Plain Text BibTeX RIS

APA Style

Li Luo, Xing Tu, Wei Liu, Xuexia Zhang, Yilin Liu, et al. (2019). Grape Seed Proanthocyanidin Extract Down-Regulates Autophagic Proteins (LC3II and Beclin-1) in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury. Clinical Medicine Research, 8(1), 16-20. https://doi.org/10.11648/j.cmr.20190801.13

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ACS Style

Li Luo; Xing Tu; Wei Liu; Xuexia Zhang; Yilin Liu, et al. Grape Seed Proanthocyanidin Extract Down-Regulates Autophagic Proteins (LC3II and Beclin-1) in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury. Clin. Med. Res. 2019, 8(1), 16-20. doi: 10.11648/j.cmr.20190801.13

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AMA Style

Li Luo, Xing Tu, Wei Liu, Xuexia Zhang, Yilin Liu, et al. Grape Seed Proanthocyanidin Extract Down-Regulates Autophagic Proteins (LC3II and Beclin-1) in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury. Clin Med Res. 2019;8(1):16-20. doi: 10.11648/j.cmr.20190801.13

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@article{10.11648/j.cmr.20190801.13,
  author = {Li Luo and Xing Tu and Wei Liu and Xuexia Zhang and Yilin Liu and Wenyan Zhao and Junhua Yang and Mengxia Wang and Jing Liu},
  title = {Grape Seed Proanthocyanidin Extract Down-Regulates Autophagic Proteins (LC3II and Beclin-1) in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury},
  journal = {Clinical Medicine Research},
  volume = {8},
  number = {1},
  pages = {16-20},
  doi = {10.11648/j.cmr.20190801.13},
  url = {https://doi.org/10.11648/j.cmr.20190801.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cmr.20190801.13},
  abstract = {Neonatal hypoxic-ischemic encephalopathy (HIE) is a severe brain disease that often leads to death or irreversible sequelae. The aim of this study was to determine the effect of a naturally active drug (grape seed proanthocyanidin extract [GSPE]) on the expression of autophagic proteins in a mouse model of neonatal hypoxic-ischemic brain injury. In this study immunofluorescence staining and Western blotting were used to detect the expression of autophagy markers (LC3II and beclin1) in the brains of neonatal mice with hypoxic-ischemic brain injuries after GSPE administration. Our study showed that GSPE pre-treatment down-regulates LC3II and beclin-1 expression, thus GSPE may be a potential drug for the treatment of HIE.},
 year = {2019}
}

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TY  - JOUR
T1  - Grape Seed Proanthocyanidin Extract Down-Regulates Autophagic Proteins (LC3II and Beclin-1) in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury
AU  - Li Luo
AU  - Xing Tu
AU  - Wei Liu
AU  - Xuexia Zhang
AU  - Yilin Liu
AU  - Wenyan Zhao
AU  - Junhua Yang
AU  - Mengxia Wang
AU  - Jing Liu
Y1  - 2019/03/25
PY  - 2019
N1  - https://doi.org/10.11648/j.cmr.20190801.13
DO  - 10.11648/j.cmr.20190801.13
T2  - Clinical Medicine Research
JF  - Clinical Medicine Research
JO  - Clinical Medicine Research
SP  - 16
EP  - 20
PB  - Science Publishing Group
SN  - 2326-9057
UR  - https://doi.org/10.11648/j.cmr.20190801.13
AB  - Neonatal hypoxic-ischemic encephalopathy (HIE) is a severe brain disease that often leads to death or irreversible sequelae. The aim of this study was to determine the effect of a naturally active drug (grape seed proanthocyanidin extract [GSPE]) on the expression of autophagic proteins in a mouse model of neonatal hypoxic-ischemic brain injury. In this study immunofluorescence staining and Western blotting were used to detect the expression of autophagy markers (LC3II and beclin1) in the brains of neonatal mice with hypoxic-ischemic brain injuries after GSPE administration. Our study showed that GSPE pre-treatment down-regulates LC3II and beclin-1 expression, thus GSPE may be a potential drug for the treatment of HIE.
VL  - 8
IS  - 1
ER  -

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