Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients

Xu Fei; Qiao Wanhai; Wang Liming

doi:doi:10.11648/j.cmr.20180703.14

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Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients

Xu Fei, Qiao Wanhai, Wang Liming

Published in Clinical Medicine Research (Volume 7, Issue 3)

Received: 5 June 2018 Accepted: 12 July 2018 Published: 8 August 2018

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Abstract

To study the expression and relationship of cyclooxygenase-2(COX-2) and peroxisome proliferators activated receptor-γ (PPARγ) in peripheral blood monouclear cells (PBMCs) of multiple organ dysfunction syndrome patients. In the emergency department and intensive care unit (ICU) of our hospital, 60 MODS patients were divided into three groups with 20 cases in each group. They were MODS1 group (APACHE II score 0-10 points), MODS 2 groups (APACHE II score 11-20 points), MODS 3 groups (APACHE II score> 20 points) and control groups of 20 healthy subjects. Venous blood were sampled 1d, 3d, 6d after diagnosis of MODS, reverse transcriptase -polymerase chain reaction (RT-PCR) were used to detect the expression of PPARγ, COX-2 in PBMCs. The expression of PPARγ, COX-2 in the same period were compared between the MODS groups and the healthy control group. The study fond that the expression of COX-2 and PPARγ were low in vehicle control group. In MODS1 group the expression of PPARγ and COX-2 was higher than in vehicle control group; during the treatment period, PPARγ was significantly increased (P <0.05), but the expression of COX-2 was significantly lower (P <0.05). PPARγ expression in MODS 2 group and MODS 3group was lower than that in vehicle control group, and its expression was progressively decreased during the treatment (P <0.05);in contrast, Expression of COX-2 in MODS2 and MODS3 group was higher than that in vehicle control group, and its level was progressively increased during the treatment (P <0.05). The study revealed a significant negative correlation between COX-2 and PPARγ in MODS1 group, MODS2 group and MODS3 group. (MODS1 group r=-0.761, P<0.01; MODS2 group: r=-0.782, P<0.01; MODS3 group: r=-0.791, P<0.01). There was no significant correlation in vehicle control group (r = -0.185, P >0.05). Thus, PPARγ may protect the MODS patients by repressing the expression of cox-2 therefore it can be used as an important indicator and potential target to determine the condition and treatment of MODS.

Published in	Clinical Medicine Research (Volume 7, Issue 3)
DOI	10.11648/j.cmr.20180703.14
Page(s)	75-79
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Multiple Organ Dysfunction Syndrome (MODS), Peroxisome Proliferator-Activated (PPARγ), Receptors-γ, Cyclooxygenase-2 (COX-2)

References

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APA Style

Xu Fei, Qiao Wanhai, Wang Liming. (2018). Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients. Clinical Medicine Research, 7(3), 75-79. https://doi.org/10.11648/j.cmr.20180703.14

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ACS Style

Xu Fei; Qiao Wanhai; Wang Liming. Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients. Clin. Med. Res. 2018, 7(3), 75-79. doi: 10.11648/j.cmr.20180703.14

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AMA Style

Xu Fei, Qiao Wanhai, Wang Liming. Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients. Clin Med Res. 2018;7(3):75-79. doi: 10.11648/j.cmr.20180703.14

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@article{10.11648/j.cmr.20180703.14,
  author = {Xu Fei and Qiao Wanhai and Wang Liming},
  title = {Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients},
  journal = {Clinical Medicine Research},
  volume = {7},
  number = {3},
  pages = {75-79},
  doi = {10.11648/j.cmr.20180703.14},
  url = {https://doi.org/10.11648/j.cmr.20180703.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cmr.20180703.14},
  abstract = {To study the expression and relationship of cyclooxygenase-2(COX-2) and peroxisome proliferators activated receptor-γ (PPARγ) in peripheral blood monouclear cells (PBMCs) of multiple organ dysfunction syndrome patients. In the emergency department and intensive care unit (ICU) of our hospital, 60 MODS patients were divided into three groups with 20 cases in each group. They were MODS1 group (APACHE II score 0-10 points), MODS 2 groups (APACHE II score 11-20 points), MODS 3 groups (APACHE II score> 20 points) and control groups of 20 healthy subjects. Venous blood were sampled 1d, 3d, 6d after diagnosis of MODS, reverse transcriptase -polymerase chain reaction (RT-PCR) were used to detect the expression of PPARγ, COX-2 in PBMCs. The expression of PPARγ, COX-2 in the same period were compared between the MODS groups and the healthy control group. The study fond that the expression of COX-2 and PPARγ were low in vehicle control group. In MODS1 group the expression of PPARγ and COX-2 was higher than in vehicle control group; during the treatment period, PPARγ was significantly increased (P 0.05). Thus, PPARγ may protect the MODS patients by repressing the expression of cox-2 therefore it can be used as an important indicator and potential target to determine the condition and treatment of MODS.},
 year = {2018}
}

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TY  - JOUR
T1  - Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients
AU  - Xu Fei
AU  - Qiao Wanhai
AU  - Wang Liming
Y1  - 2018/08/08
PY  - 2018
N1  - https://doi.org/10.11648/j.cmr.20180703.14
DO  - 10.11648/j.cmr.20180703.14
T2  - Clinical Medicine Research
JF  - Clinical Medicine Research
JO  - Clinical Medicine Research
SP  - 75
EP  - 79
PB  - Science Publishing Group
SN  - 2326-9057
UR  - https://doi.org/10.11648/j.cmr.20180703.14
AB  - To study the expression and relationship of cyclooxygenase-2(COX-2) and peroxisome proliferators activated receptor-γ (PPARγ) in peripheral blood monouclear cells (PBMCs) of multiple organ dysfunction syndrome patients. In the emergency department and intensive care unit (ICU) of our hospital, 60 MODS patients were divided into three groups with 20 cases in each group. They were MODS1 group (APACHE II score 0-10 points), MODS 2 groups (APACHE II score 11-20 points), MODS 3 groups (APACHE II score> 20 points) and control groups of 20 healthy subjects. Venous blood were sampled 1d, 3d, 6d after diagnosis of MODS, reverse transcriptase -polymerase chain reaction (RT-PCR) were used to detect the expression of PPARγ, COX-2 in PBMCs. The expression of PPARγ, COX-2 in the same period were compared between the MODS groups and the healthy control group. The study fond that the expression of COX-2 and PPARγ were low in vehicle control group. In MODS1 group the expression of PPARγ and COX-2 was higher than in vehicle control group; during the treatment period, PPARγ was significantly increased (P 0.05). Thus, PPARγ may protect the MODS patients by repressing the expression of cox-2 therefore it can be used as an important indicator and potential target to determine the condition and treatment of MODS.
VL  - 7
IS  - 3
ER  -

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Author Information

Xu Fei

Department of Emergency Medicine, 3201 Hospital of Xi'an Jiaotong University Health Science Center, Hanzhong, China
Qiao Wanhai

Department of Emergency Medicine, Second Affiliated Hospital of Xi'an Jiaotong University Health Science Center, Xi’an, China
Wang Liming

Department of Emergency Medicine, Second Affiliated Hospital of Xi'an Jiaotong University Health Science Center, Xi’an, China

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Plain Text BibTeX RIS

APA Style

Xu Fei, Qiao Wanhai, Wang Liming. (2018). Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients. Clinical Medicine Research, 7(3), 75-79. https://doi.org/10.11648/j.cmr.20180703.14

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ACS Style

Xu Fei; Qiao Wanhai; Wang Liming. Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients. Clin. Med. Res. 2018, 7(3), 75-79. doi: 10.11648/j.cmr.20180703.14

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AMA Style

Xu Fei, Qiao Wanhai, Wang Liming. Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients. Clin Med Res. 2018;7(3):75-79. doi: 10.11648/j.cmr.20180703.14

Copy | Download

@article{10.11648/j.cmr.20180703.14,
  author = {Xu Fei and Qiao Wanhai and Wang Liming},
  title = {Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients},
  journal = {Clinical Medicine Research},
  volume = {7},
  number = {3},
  pages = {75-79},
  doi = {10.11648/j.cmr.20180703.14},
  url = {https://doi.org/10.11648/j.cmr.20180703.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cmr.20180703.14},
  abstract = {To study the expression and relationship of cyclooxygenase-2(COX-2) and peroxisome proliferators activated receptor-γ (PPARγ) in peripheral blood monouclear cells (PBMCs) of multiple organ dysfunction syndrome patients. In the emergency department and intensive care unit (ICU) of our hospital, 60 MODS patients were divided into three groups with 20 cases in each group. They were MODS1 group (APACHE II score 0-10 points), MODS 2 groups (APACHE II score 11-20 points), MODS 3 groups (APACHE II score> 20 points) and control groups of 20 healthy subjects. Venous blood were sampled 1d, 3d, 6d after diagnosis of MODS, reverse transcriptase -polymerase chain reaction (RT-PCR) were used to detect the expression of PPARγ, COX-2 in PBMCs. The expression of PPARγ, COX-2 in the same period were compared between the MODS groups and the healthy control group. The study fond that the expression of COX-2 and PPARγ were low in vehicle control group. In MODS1 group the expression of PPARγ and COX-2 was higher than in vehicle control group; during the treatment period, PPARγ was significantly increased (P 0.05). Thus, PPARγ may protect the MODS patients by repressing the expression of cox-2 therefore it can be used as an important indicator and potential target to determine the condition and treatment of MODS.},
 year = {2018}
}

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TY  - JOUR
T1  - Expression and relationship of PPARγ and COX-2 in Peripheral Blood Mononuclear Cells of Multiple Organ Dysfunction Syndrome Patients
AU  - Xu Fei
AU  - Qiao Wanhai
AU  - Wang Liming
Y1  - 2018/08/08
PY  - 2018
N1  - https://doi.org/10.11648/j.cmr.20180703.14
DO  - 10.11648/j.cmr.20180703.14
T2  - Clinical Medicine Research
JF  - Clinical Medicine Research
JO  - Clinical Medicine Research
SP  - 75
EP  - 79
PB  - Science Publishing Group
SN  - 2326-9057
UR  - https://doi.org/10.11648/j.cmr.20180703.14
AB  - To study the expression and relationship of cyclooxygenase-2(COX-2) and peroxisome proliferators activated receptor-γ (PPARγ) in peripheral blood monouclear cells (PBMCs) of multiple organ dysfunction syndrome patients. In the emergency department and intensive care unit (ICU) of our hospital, 60 MODS patients were divided into three groups with 20 cases in each group. They were MODS1 group (APACHE II score 0-10 points), MODS 2 groups (APACHE II score 11-20 points), MODS 3 groups (APACHE II score> 20 points) and control groups of 20 healthy subjects. Venous blood were sampled 1d, 3d, 6d after diagnosis of MODS, reverse transcriptase -polymerase chain reaction (RT-PCR) were used to detect the expression of PPARγ, COX-2 in PBMCs. The expression of PPARγ, COX-2 in the same period were compared between the MODS groups and the healthy control group. The study fond that the expression of COX-2 and PPARγ were low in vehicle control group. In MODS1 group the expression of PPARγ and COX-2 was higher than in vehicle control group; during the treatment period, PPARγ was significantly increased (P 0.05). Thus, PPARγ may protect the MODS patients by repressing the expression of cox-2 therefore it can be used as an important indicator and potential target to determine the condition and treatment of MODS.
VL  - 7
IS  - 3
ER  -

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