The objective of this study was to characterize the immunohistochemical localization of Na+/H+ exchanger isoforms (NHE1 and NHE3) in the rumen epithelium of Sheep after changing the diet from hay (ad libitum) to a mixed hay/concentrate diet. A total of 24 sheep were fed mixed hay/concentrate for different periods ranging from 0 weeks (control; hay ad libitum) to 12 weeks (1-1.5 kg hay plus 780 g concentrate per day in two equal portions). NHE3-immunostaining was found to be more intense at both stratum granulosum (deep layer) and stratum spinosum (superficial layer), with decreasing intensity through stratum spinosum (deep or suprabasal layer) and stratum basal. Stratum corneum was negative. Distribution of NHE3 isoform was different within the different strata. In stratum granulosum and stratum spinosum (superficial layer), NHE3 isoform was distributed predominant at the apical surface /membrane of the cells. Meanwhile, in both stratum spinosum (deep layer) and stratum basale, intracellular NHE3 isoform was predominantly. The degree of antibody reaction was weak in hay-fed sheep and in all concentrate-fed groups, except in 2 and 4 weeks concentrate-fed groups, in which the degree of the antibody reaction was moderate and strong, respectively. NHE1 isoform was not detected in the sheep-rumen epithelium.
| Published in | Animal and Veterinary Sciences (Volume 2, Issue 6) |
| DOI | 10.11648/j.avs.20140206.18 |
| Page(s) | 208-212 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
Sheep, Rumen Epithelium, NHE1, NHE3, Feed
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APA Style
Rasha S. Ahmed, Holger Martens, Christoph Muelling. (2014). Diet-Dependent Rumen Epithelial NHE1 and NHE3 Expression in Sheep. Animal and Veterinary Sciences, 2(6), 208-212. https://doi.org/10.11648/j.avs.20140206.18
ACS Style
Rasha S. Ahmed; Holger Martens; Christoph Muelling. Diet-Dependent Rumen Epithelial NHE1 and NHE3 Expression in Sheep. Anim. Vet. Sci. 2014, 2(6), 208-212. doi: 10.11648/j.avs.20140206.18
AMA Style
Rasha S. Ahmed, Holger Martens, Christoph Muelling. Diet-Dependent Rumen Epithelial NHE1 and NHE3 Expression in Sheep. Anim Vet Sci. 2014;2(6):208-212. doi: 10.11648/j.avs.20140206.18
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Download@article{10.11648/j.avs.20140206.18,
author = {Rasha S. Ahmed and Holger Martens and Christoph Muelling},
title = {Diet-Dependent Rumen Epithelial NHE1 and NHE3 Expression in Sheep},
journal = {Animal and Veterinary Sciences},
volume = {2},
number = {6},
pages = {208-212},
doi = {10.11648/j.avs.20140206.18},
url = {https://doi.org/10.11648/j.avs.20140206.18},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20140206.18},
abstract = {The objective of this study was to characterize the immunohistochemical localization of Na+/H+ exchanger isoforms (NHE1 and NHE3) in the rumen epithelium of Sheep after changing the diet from hay (ad libitum) to a mixed hay/concentrate diet. A total of 24 sheep were fed mixed hay/concentrate for different periods ranging from 0 weeks (control; hay ad libitum) to 12 weeks (1-1.5 kg hay plus 780 g concentrate per day in two equal portions). NHE3-immunostaining was found to be more intense at both stratum granulosum (deep layer) and stratum spinosum (superficial layer), with decreasing intensity through stratum spinosum (deep or suprabasal layer) and stratum basal. Stratum corneum was negative. Distribution of NHE3 isoform was different within the different strata. In stratum granulosum and stratum spinosum (superficial layer), NHE3 isoform was distributed predominant at the apical surface /membrane of the cells. Meanwhile, in both stratum spinosum (deep layer) and stratum basale, intracellular NHE3 isoform was predominantly. The degree of antibody reaction was weak in hay-fed sheep and in all concentrate-fed groups, except in 2 and 4 weeks concentrate-fed groups, in which the degree of the antibody reaction was moderate and strong, respectively. NHE1 isoform was not detected in the sheep-rumen epithelium.},
year = {2014}
}
TY - JOUR T1 - Diet-Dependent Rumen Epithelial NHE1 and NHE3 Expression in Sheep AU - Rasha S. Ahmed AU - Holger Martens AU - Christoph Muelling Y1 - 2014/12/19 PY - 2014 N1 - https://doi.org/10.11648/j.avs.20140206.18 DO - 10.11648/j.avs.20140206.18 T2 - Animal and Veterinary Sciences JF - Animal and Veterinary Sciences JO - Animal and Veterinary Sciences SP - 208 EP - 212 PB - Science Publishing Group SN - 2328-5850 UR - https://doi.org/10.11648/j.avs.20140206.18 AB - The objective of this study was to characterize the immunohistochemical localization of Na+/H+ exchanger isoforms (NHE1 and NHE3) in the rumen epithelium of Sheep after changing the diet from hay (ad libitum) to a mixed hay/concentrate diet. A total of 24 sheep were fed mixed hay/concentrate for different periods ranging from 0 weeks (control; hay ad libitum) to 12 weeks (1-1.5 kg hay plus 780 g concentrate per day in two equal portions). NHE3-immunostaining was found to be more intense at both stratum granulosum (deep layer) and stratum spinosum (superficial layer), with decreasing intensity through stratum spinosum (deep or suprabasal layer) and stratum basal. Stratum corneum was negative. Distribution of NHE3 isoform was different within the different strata. In stratum granulosum and stratum spinosum (superficial layer), NHE3 isoform was distributed predominant at the apical surface /membrane of the cells. Meanwhile, in both stratum spinosum (deep layer) and stratum basale, intracellular NHE3 isoform was predominantly. The degree of antibody reaction was weak in hay-fed sheep and in all concentrate-fed groups, except in 2 and 4 weeks concentrate-fed groups, in which the degree of the antibody reaction was moderate and strong, respectively. NHE1 isoform was not detected in the sheep-rumen epithelium. VL - 2 IS - 6 ER -
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