The ligands of many orphan G protein-coupled receptors (GPCRs) remain to be identified, in both vertebrates and invertebrates, such as Drosophila melanogaster. Identification of their cognate ligands is critical for understanding the function and regulation of such GPCRs. Indeed, the discovery of bioactive peptides that bind GPCRs has enhanced our understanding of the mechanisms underlying many physiological processes. Here, we identified five endogenous ligands of the Drosophila orphan GPCRs, using functional assays and reverse pharmacological techniques. dRYamide-1 and -2 were found to be paired with the Drosophila neuropeptide Y (NPY)-like receptor (CG5811). Both dRYamide-1 and -2 contain a C-terminal RYamide. In vertebrates, RYamide motifs are found in NPY-family peptides. dRYamides were found to modulate feeding motivation in flies. These results suggest that deorphanizing the Drosophila orphan GPCRs might facilitate the elucidation of various physiological functions and identification of the ligands of orphan GPCRs in mammals.
| Published in |
American Journal of Life Sciences (Volume 3, Issue 3-2)
This article belongs to the Special Issue Biology and Medicine of Peptide and Steroid Hormones |
| DOI | 10.11648/j.ajls.s.2015030302.12 |
| Page(s) | 3-7 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Novel Bioactive Peptide, Orphan GPCR, Drosophila
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APA Style
Takanori Ida, Eri Iwamoto, Takahiro Sato, Masayasu Kojima. (2015). Identification and Application of Drosophila Novel Bioactive Peptides dRYamides. American Journal of Life Sciences, 3(3-2), 3-7. https://doi.org/10.11648/j.ajls.s.2015030302.12
ACS Style
Takanori Ida; Eri Iwamoto; Takahiro Sato; Masayasu Kojima. Identification and Application of Drosophila Novel Bioactive Peptides dRYamides. Am. J. Life Sci. 2015, 3(3-2), 3-7. doi: 10.11648/j.ajls.s.2015030302.12
AMA Style
Takanori Ida, Eri Iwamoto, Takahiro Sato, Masayasu Kojima. Identification and Application of Drosophila Novel Bioactive Peptides dRYamides. Am J Life Sci. 2015;3(3-2):3-7. doi: 10.11648/j.ajls.s.2015030302.12
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Download@article{10.11648/j.ajls.s.2015030302.12,
author = {Takanori Ida and Eri Iwamoto and Takahiro Sato and Masayasu Kojima},
title = {Identification and Application of Drosophila Novel Bioactive Peptides dRYamides},
journal = {American Journal of Life Sciences},
volume = {3},
number = {3-2},
pages = {3-7},
doi = {10.11648/j.ajls.s.2015030302.12},
url = {https://doi.org/10.11648/j.ajls.s.2015030302.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2015030302.12},
abstract = {The ligands of many orphan G protein-coupled receptors (GPCRs) remain to be identified, in both vertebrates and invertebrates, such as Drosophila melanogaster. Identification of their cognate ligands is critical for understanding the function and regulation of such GPCRs. Indeed, the discovery of bioactive peptides that bind GPCRs has enhanced our understanding of the mechanisms underlying many physiological processes. Here, we identified five endogenous ligands of the Drosophila orphan GPCRs, using functional assays and reverse pharmacological techniques. dRYamide-1 and -2 were found to be paired with the Drosophila neuropeptide Y (NPY)-like receptor (CG5811). Both dRYamide-1 and -2 contain a C-terminal RYamide. In vertebrates, RYamide motifs are found in NPY-family peptides. dRYamides were found to modulate feeding motivation in flies. These results suggest that deorphanizing the Drosophila orphan GPCRs might facilitate the elucidation of various physiological functions and identification of the ligands of orphan GPCRs in mammals.},
year = {2015}
}
TY - JOUR T1 - Identification and Application of Drosophila Novel Bioactive Peptides dRYamides AU - Takanori Ida AU - Eri Iwamoto AU - Takahiro Sato AU - Masayasu Kojima Y1 - 2015/05/06 PY - 2015 N1 - https://doi.org/10.11648/j.ajls.s.2015030302.12 DO - 10.11648/j.ajls.s.2015030302.12 T2 - American Journal of Life Sciences JF - American Journal of Life Sciences JO - American Journal of Life Sciences SP - 3 EP - 7 PB - Science Publishing Group SN - 2328-5737 UR - https://doi.org/10.11648/j.ajls.s.2015030302.12 AB - The ligands of many orphan G protein-coupled receptors (GPCRs) remain to be identified, in both vertebrates and invertebrates, such as Drosophila melanogaster. Identification of their cognate ligands is critical for understanding the function and regulation of such GPCRs. Indeed, the discovery of bioactive peptides that bind GPCRs has enhanced our understanding of the mechanisms underlying many physiological processes. Here, we identified five endogenous ligands of the Drosophila orphan GPCRs, using functional assays and reverse pharmacological techniques. dRYamide-1 and -2 were found to be paired with the Drosophila neuropeptide Y (NPY)-like receptor (CG5811). Both dRYamide-1 and -2 contain a C-terminal RYamide. In vertebrates, RYamide motifs are found in NPY-family peptides. dRYamides were found to modulate feeding motivation in flies. These results suggest that deorphanizing the Drosophila orphan GPCRs might facilitate the elucidation of various physiological functions and identification of the ligands of orphan GPCRs in mammals. VL - 3 IS - 3-2 ER -
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