The effect of Nigella sativa (black cumin) seed extracts supplemented diets at 2%, 4% and 6% doses with common diet as control (0%) fed to common carp, Cyprinus carpio against Pseudomonas fluorescens was investigated critically on weeks 1, 2 and 4 as immunomodulator. Immunological factors including bactericidal activity and phagocytic action were examined along with the disease resistance. The outcomes of the research recommend 4% dose of N. sativa oil enriched diet as an effective immune response and disease resistance agent for C. carpio against P. fluorescens although further investigation will facilitate to optimize the precise quantity comparing with the weight of fish body.
Published in | American Journal of Life Sciences (Volume 4, Issue 3) |
DOI | 10.11648/j.ajls.20160403.14 |
Page(s) | 87-92 |
Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
Nigella sativa, Common Carp, Pseudomonas fluorescens, Immune Response
[1] | Vandeputte, M., 2003. Selective breeding of quantitative traits in the common carp (Cyprinus carpio): a review. Aquat Living Resour, 16 (5): 399-407. |
[2] | Froese, R. and Pauly, D., 2011. FishBase. World Wide Web electronic publication, available at: www.fishbase.org/summary/speciessummary.php; |
[3] | FAO Fisheries and Aquaculture Department, 2015. Cultured Aquatic Species Information Programme: Cyprinus carpio (Linnaeus, 1758). |
[4] | Chowdhury, M. B. R. and Baqui, M. A., 1997. Bacterial flora in farmed carp (Labeo rohita) in Bangladesh. In: Disease in Asian Aquaculture III (eds. T. Flegel and I. H. Mac Rae), Fish Health Section, Asian Fisheries Society, Manila. p. 101-105. |
[5] | Khatun, H., Hossain, M. D., Jahan, S. N. and Khanom, D. A., 2011. Bacterial infestation in different fish at Rajshahi. J Sci Foundation, 9 (1 & 2): 77-84. |
[6] | Harikrishnan, R., Balasundaram, C. and Heo, M. S., 2011. Impact of plant products on innate and adaptive immune system of cultured finfish and shellfish. Aquaculture, 317: 1-15. |
[7] | Robertsen, B., 1999. Modulation of the non-specific defense of fish by structurally conserved microbial polymers. Fish & Shellfish Immunol, 9: 269–290. |
[8] | Rao, Y. V., Das, B. K., Jyotyrmayee, P. and Chakrabarti, R., 2006. Effect of Achyranthes aspera on the immunity and survival of Labeo rohita infected with Aeromonas hydrophila. Fish & Shellfish Immunol, 20: 263–273. |
[9] | Awad, E., Austin, D. and Lyndon, A. R., 2013. Effect of black cumin seed oil (Nigella sativa) and nettle extract (Quercetin) on enhancement of immunity in rainbow trout, Oncorhynchus mykiss (Walbaum). Aquaculture, 388-391: 193-197. |
[10] | Nya, E. J. and Austin, B., 2009. Use of garlic, Allium sativum, to control Aeromonas hydrophila infection in rainbow trout, Oncorhynchus mykiss (Walbaum). J Fish Dis, 32: 963–970. |
[11] | Awad, E. and Austin, B., 2010. Use of lupin, Lupinus perennis, mango, Mangifera indica, and stinging nettle, Urtica dioica, as feed additives to prevent Aeromonas hydrophila infection in rainbow trout, Oncorhynchus mykiss (Walbaum). J. Fish Dis. 33 (5): 413–420. |
[12] | Dügenci, S. K., Arda, N. and Candan, A., 2003. Some medicinal plants as immunostimulant for fish. J Ethnopharmacol. 88: 99–106. |
[13] | Christybapita, D., Divyagnaneswari, M. and Michael, R. D., 2007. Oral administration of Eclipta alba leaf aqueous extract enhances the non-specific immune responses and disease resistance of Oreochromis mossambicus. Fish Shellfish Immunol, 23: 840–852. |
[14] | Ardo, L., Yin, G., Xu, P., Varadi, L., Szigeti, G., Jeney, Z. and Jeney, G., 2008. Chinese herbs (Astragalus membranaceus and Lonicera japonica) and boron enhance the nonspecific immune response of Nile tilapia (Oreochromis niloticus) and resistance against Aeromonas hydrophila. Aquaculture, 275 (1-4): 26–33. |
[15] | Xie, J., Liu, B., Zhou, Q., Su, Y., He, Y., Pan, L., Ge, X. and Xu, P., 2008. Effects of anthraquinone extract from rhubarb Rheum officinale Bail on the crowding stress response and growth of common carp Cyprinus carpio var. Jian. Aquaculture, 281: 5–11. |
[16] | Jian, J. and Wu, Z., 2003. Effects of traditional Chinese medicine on nonspecific immunity and disease resistance of large yellow croaker, Pseudosciaena crocea (Richardson). Aquaculture, 218: 1–9. |
[17] | Jian, J. and Wu, Z., 2004. Influence of traditional Chinese medicine on non specific immunity of Jian Carp (Cyprinus carpio var. Jian). Fish & Shellfish Immunol, 16: 185–191. |
[18] | Public Health Agency of Canada, 2011. Pseudomonas spp.: Pathogen safety data sheet Infectious Substances. |
[19] | Tryfinopoulou, P., Tsakalidou, E. and Nychas, G. J. E., 2002. Characterization of Pseudomonas spp. Associated with spoilage of gilt-head sea bream stored under various conditions. Appl Environ Microbiol, 68 (1): 65-72. |
[20] | Franzetti, L. and Scarpellini, M., 2007. Characterization of Pseudomonas spp. isolated from foods. Ann. Microbiol., 57 (1): 39-47. |
[21] | Soesanto, L., Mugiastuti, E. and Rahayuniati, R. F., 2007. Biochemical Characteristic of Pseudomonas fluorescens P60. Dept. of Plant Pests and Diseases, Faculty of Agcirulture, Jenderal Soedirman University, P. O. Box 125, Purwokerto 53101. |
[22] | Couillerot, O., Prigent-Combaret, C., Caballero-Mellado, J. and Moe¨ nne-Loccoz, Y., 2008. Pseudomonas fluorescens and closely-related fluorescent pseudomonads as biocontrol agents of soil-borne phytopathogens. The Society for Applied Microbiology, Lett Appl Microbiol, 48: 505–512. |
[23] | Khan M. A., 1999. Chemical composition and medicinal properties of Nigella sativa Linn. Inflammopharmacology, 7 (1): 15-35. |
[24] | Ramadan, M. F., 2007. Nutritional value, functional properties and nutraceutical applications of black cumin (Nigella sativa L.): an overview. Int J Food Sci Technol, 42: 1208–1218. |
[25] | Tembhurne, S. V., Feroz, S., More, B. H. and Sakarkar, D. M., 2014. A review on therapeutic potential of Nigella sativa (kalonji) seeds. J Med Plant Res, 8 (3): 167-177. |
[26] | Cooper and Gunn, 2005. Tutorial pharmacy. Extraction Methods. CBS Publishers & Distributors, New Delhi, India, p. 257-259. |
[27] | Amend, D., 1981. Potency of testing of fish vaccines. Dev. Biol. Stand., 49: 447-454. |
[28] | Maggon, K., 2005. Best selling human medicine 2002–2004. Drug Dis Today, 11, 739–742. |
[29] | El-Kadi, A., Kandil, O. and Tabuni, A. M., 1987. Nigella sativa and cell mediated immunity. Archives of AIDS Research. 1: 232-233. |
[30] | Hedaya, S. A., 1995. Effect of Nigella sativa (black seeds) extract on some haematological and biochemical parameters in rats. Alex J Vet Sci, 11: 95–99. |
[31] | Mandour, A., Mahmoud, K., Abou El-Wafa, A., El- Agamy, E. and Ragab, O., 1995. Effect of aflatoxin and Nigella sativa seeds on serum proteins and its electrophoretic patterns in White Pekin ducklings. 1st Egyptian Hungarian Poultry Conference, Alexandria, Egypt. |
[32] | Abdel-Ghaffar, F. A., El-Feki, M. A., Faheem, H. I. and Gad, M. A., 2003. Potentiating effects of some natural products on the common carp immune system against Pseudomonas florescence. Egypt. Aquat. Biol. Fish., 7: 23–47. |
[33] | Taha, N., Korshom, M., Mandour, A., El-Bahr, S. M. and El-Sirafy, S., 2007. Effect of some immunostimulentant agents on serum proteins and amino acids patterns of Japanese qyils. Alex J Vet Med, 25: 47-53. |
[34] | Harikrishnan, R., Kim, J. S., Balasundaram, C. and Heo, M. S., 2012. Protection of Vibrio harveyi infection through dietary administration of Pueraria thunbergiana in kelp grouper, Epinephelus bruneus. Aquaculture, 324-325: 27-32. |
[35] | Harikrishnan, R., Rani, M. N. and Balasundaram, C., 2003. Hematological and biochemical parameters in common carp, Cyprinus carpio, following herbal treatment for Aeromonas hydrophila infection. Aquaculture, 221 (1-4): 41-50. |
[36] | Grayson, T. H., Williams, R. J., Wrathmell, A. B., Munn, C. B. and Harris, J. E., 1987. Effects of immunopotentiating agents in the immune response of rainbow trout, Salmo gairdnen kchardson, to ERM vaccine. J Fish Biol, 31: 195–202. |
[37] | Chen, X., Wu, Z., Yin, J. and Li, L., 2003. Effects of four species of herbs on immune function of Carassius auratus gibelio. J. Fish. Sci. China, 10: 36–40. |
[38] | Yuan, C., Li, D., Chen, W., Sun, F., Wu, G., Gong, Y., Tang, J., Shen, M. and Han, X., 2007. Administration of a herbal immunoregulation mixture enhances some immune parameters in carp (Cyprinus carpuio). Fish Physiol Biochem, 33: 93–101. |
[39] | Yin, G., Ardo, L., Thompson, K. D., Adams, A., Jeney, Z. and Jeney, G., 2009. Chinese herbs (Astragalus radix and Ganoderma lucidum) enhance immune response of carp, Cyprinus carpio, and protection against Aeromonas hydrophila. Fish & Shellfish Immunol, 26: 140–145. |
[40] | Ngugi, C. C., Oyoo-Okoth, E., Mugo-Bundi, J., Orina, P. S., Chemoiwa, E. J. and Aloo, P. A., 2015. Effects of dietary administration of stinging nettle (Urtica dioica) on the growth performance, biochemical, hematological and immunological parameters in juvenile and adult Victoria Labeo (Labeo victorianus) challenged with Aeromonas hydrophila. Fish & Shellfish Immunol, 44: 533-541. |
[41] | Mohamad, S. and Abasali, H., 2010. Effect of plant extracts supplemented diets on immunity and resistance to Aeromonas hydrophila in common carp (Cyprinus carpio). Agri J, 5 (2): 119-127. |
[42] | Yımaz, S., Ergün, S. and Soytaş, N., 2013. Dietary supplementation of cumin (Cuminum cyminum) preventing streptococcal disease during first-feeding of Mozambique tilapia (Oreochromis mossambicus). J BioSci Biotechnol, 2 (2): 117-124. |
[43] | Harikrishnan, R., Kim, J. S., Kim, M. C., Balasundaram, C. and Heo, M. S., 2011a. Prunella vulgaris enhances the non-specific immune response and disease resistance of Paralichthys olivaceus against Uronema marinum. Aquaculture, 318: 61-66. |
APA Style
Shoumo Khondoker, Md. Mer Mosharraf Hossain, Md. Hasan-Uj-Jaman, Md. Eftakher Alam, Md. Farid Uz Zaman, et al. (2016). Effect of Nigella sativa (Black Cumin Seed) to Enhance the Immunity of Common Carp (Cyprinus carpio) Against Pseudomonas fluorescens. American Journal of Life Sciences, 4(3), 87-92. https://doi.org/10.11648/j.ajls.20160403.14
ACS Style
Shoumo Khondoker; Md. Mer Mosharraf Hossain; Md. Hasan-Uj-Jaman; Md. Eftakher Alam; Md. Farid Uz Zaman, et al. Effect of Nigella sativa (Black Cumin Seed) to Enhance the Immunity of Common Carp (Cyprinus carpio) Against Pseudomonas fluorescens. Am. J. Life Sci. 2016, 4(3), 87-92. doi: 10.11648/j.ajls.20160403.14
AMA Style
Shoumo Khondoker, Md. Mer Mosharraf Hossain, Md. Hasan-Uj-Jaman, Md. Eftakher Alam, Md. Farid Uz Zaman, et al. Effect of Nigella sativa (Black Cumin Seed) to Enhance the Immunity of Common Carp (Cyprinus carpio) Against Pseudomonas fluorescens. Am J Life Sci. 2016;4(3):87-92. doi: 10.11648/j.ajls.20160403.14
@article{10.11648/j.ajls.20160403.14, author = {Shoumo Khondoker and Md. Mer Mosharraf Hossain and Md. Hasan-Uj-Jaman and Md. Eftakher Alam and Md. Farid Uz Zaman and Nazia Tabassum}, title = {Effect of Nigella sativa (Black Cumin Seed) to Enhance the Immunity of Common Carp (Cyprinus carpio) Against Pseudomonas fluorescens}, journal = {American Journal of Life Sciences}, volume = {4}, number = {3}, pages = {87-92}, doi = {10.11648/j.ajls.20160403.14}, url = {https://doi.org/10.11648/j.ajls.20160403.14}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20160403.14}, abstract = {The effect of Nigella sativa (black cumin) seed extracts supplemented diets at 2%, 4% and 6% doses with common diet as control (0%) fed to common carp, Cyprinus carpio against Pseudomonas fluorescens was investigated critically on weeks 1, 2 and 4 as immunomodulator. Immunological factors including bactericidal activity and phagocytic action were examined along with the disease resistance. The outcomes of the research recommend 4% dose of N. sativa oil enriched diet as an effective immune response and disease resistance agent for C. carpio against P. fluorescens although further investigation will facilitate to optimize the precise quantity comparing with the weight of fish body.}, year = {2016} }
TY - JOUR T1 - Effect of Nigella sativa (Black Cumin Seed) to Enhance the Immunity of Common Carp (Cyprinus carpio) Against Pseudomonas fluorescens AU - Shoumo Khondoker AU - Md. Mer Mosharraf Hossain AU - Md. Hasan-Uj-Jaman AU - Md. Eftakher Alam AU - Md. Farid Uz Zaman AU - Nazia Tabassum Y1 - 2016/06/17 PY - 2016 N1 - https://doi.org/10.11648/j.ajls.20160403.14 DO - 10.11648/j.ajls.20160403.14 T2 - American Journal of Life Sciences JF - American Journal of Life Sciences JO - American Journal of Life Sciences SP - 87 EP - 92 PB - Science Publishing Group SN - 2328-5737 UR - https://doi.org/10.11648/j.ajls.20160403.14 AB - The effect of Nigella sativa (black cumin) seed extracts supplemented diets at 2%, 4% and 6% doses with common diet as control (0%) fed to common carp, Cyprinus carpio against Pseudomonas fluorescens was investigated critically on weeks 1, 2 and 4 as immunomodulator. Immunological factors including bactericidal activity and phagocytic action were examined along with the disease resistance. The outcomes of the research recommend 4% dose of N. sativa oil enriched diet as an effective immune response and disease resistance agent for C. carpio against P. fluorescens although further investigation will facilitate to optimize the precise quantity comparing with the weight of fish body. VL - 4 IS - 3 ER -