Antimicrobial Inhibition on Zoonotic Bacterial Escherichia coli O157: H7 as a Cause of Food Borne Disease

Lucia Winata Muslimin; Abdul Wahid Jamaluddin; Ressy Dwiyanti; Mochammad Hatta

doi:doi:10.11648/j.ajbls.20140206.15

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Antimicrobial Inhibition on Zoonotic Bacterial Escherichia coli O157: H7 as a Cause of Food Borne Disease

Lucia Winata Muslimin, Abdul Wahid Jamaluddin, Ressy Dwiyanti, Mochammad Hatta

Published in American Journal of Biomedical and Life Sciences (Volume 2, Issue 6)

Received: 23 December 2014 Accepted: 31 December 2014 Published: 8 January 2015

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Abstract

This study aims to accelerate zoonosis control system, secure food safety and improve the environmental quality. Meat samples, swab and water were acquired from five regions in South Sulawesi, Indonesia. The samples were implanted to bacterial growth medium inside the ice-box and carried to the lab to be inoculated with Nutrient Agar, Eosin Methylen Blue Agar Sorbitol Mac Conkey Agar (SMAC). IMVIC test, Biochemical Test and pathogenic test with blood Agar from the suspected Escherichia coli O157H7 result, was followed by PCR test to genetically identify the bacteria. The result was then examined for sensitivity test with antibiotics: Imipenem, Tetracycline, Erytromycin, Levofloxacine, Amoxycilin, Chloramphenicol and Ciprofloxacine. Among 117 samples. 43 was assumed positive with culture method, 12 was assumed positive with PCR E.coli O157H7.The most sensitive antibiotics, Imipenem, Ciprofloxacine and levofloxacine.

Published in	American Journal of Biomedical and Life Sciences (Volume 2, Issue 6)
DOI	10.11648/j.ajbls.20140206.15
Page(s)	163-166
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Antimicrobial Inhibition, Food Borne Disease, E.coli

References

[1]	Ateba, Collins Njie,MosesMbewe. 2014.Genotypic Characterization of Escherichia coli O157:H7 Isolates from Different Sources in the North-West Province, South Africa, Using Enterobacterial Repetitive Intergenic Consensus PCR Analysis.Int J Mol Sci. 2014; 15(6): 9735–9747
[2]	Muslimin L. 2012. Escherichia coli contaminated from animal product, water supply and fresh vegetables from traditional Market. Private research. LLPM Journal 219: 1-9.
[3]	Cruickshank R, 1968. Medical Microbiology: A Guide to theLaboratory Diagnosis and Control of Infection, Edinburgh:E& S Livingstone Ltd.
[4]	Hatta M, Andi Rofian Sultan, RessyDwiyanti, et al. 2014. The Reproducibility of the Multiplex RAPD-PCR assay in Genotyping of Mycobacterium tuberculosis isolates from Sulawesi, Indonesia. American Journal of Clinical and Experimental Medicine 2014. Vol. 2, No. 2: 14-21. DOI: 10.11648/j.ajcem.20140202.
[5]	Nicole J. Morin, Zhilong Gong, and Xing-Fang Li. 2004. Reverse. Transcription-Multiplex PCR Assay for Simultaneous Detection of Escherichia coliO157:H7, Vibrio choleraeO1, and Salmonella Typhi. Clinical Chemistry 50:11:2037–2044.
[6]	National Committee for Clinical Laboratory Standards (NCCLS). 2004. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals.Approved standard M13-A. Wayne: NCCLS
[7]	Linda Larsen, 2013. Food Poisoning at Federico’s is Largest U.S. E. coli Outbreak in Years. Food Poisoning Bulletin. 32-45
[8]	Linda Larsen, 2013. McBee Dairy Farm in TN Linked to E. coli O157:H7 Outbreak.Food Poisoning Bulletin.21-29
[9]	CDC.2014.Multistate Outbreak of Shiga toxin-producing Escherichia coliO157:H7 Infections Linked to Ground Beef .http://www.cdc.gov/ecoli/2014/O157H7-05-14/index.html
[10]	Turner, Marian.2011. Spread of rare Escherichia coli strain raises questions over surveillance of infectious diseases. Microbe outbreak panics Europe. http://www.nature.com/news/2011/110607/full/474137a.htm
[11]	White, D.G., Zhao, S., Simjee, S., Wagner, D.D. and McDermott, P.F. 2002.Antimicrobial resistance of foodborne pathogens.Microbes and Infection, 4: 405-412
[12]	Perna NT, Ill GP, Burland V, Mau B, Glasner JD, Rose DJ, Mayhew GF, Evans PS, Gregor J, Kirkpatrick HA. 2001. Genome sequence of enterohaemorrhagicEscherichiacoli O157:H7. Nature 409:529-31
[13]	Alam, M. J. And L, Zurek. 2004. Association of Escherichia coli O157:H7 with houseflies on a cattle farm. Applied and Environmental Microbiology, 70 (12), 7578-7580
[14]	Feng P, Sandlin RC, Park CH, Wilson RA, Nishibuchi M. 1998. Identification of a rough strain of Escherichiacoli O157:H7 that produces no detectable O157 antigen. J Clin.Microbiol 36(8):2339-2341

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APA Style

Lucia Winata Muslimin, Abdul Wahid Jamaluddin, Ressy Dwiyanti, Mochammad Hatta. (2015). Antimicrobial Inhibition on Zoonotic Bacterial Escherichia coli O157: H7 as a Cause of Food Borne Disease. American Journal of Biomedical and Life Sciences, 2(6), 163-166. https://doi.org/10.11648/j.ajbls.20140206.15

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ACS Style

Lucia Winata Muslimin; Abdul Wahid Jamaluddin; Ressy Dwiyanti; Mochammad Hatta. Antimicrobial Inhibition on Zoonotic Bacterial Escherichia coli O157: H7 as a Cause of Food Borne Disease. Am. J. Biomed. Life Sci. 2015, 2(6), 163-166. doi: 10.11648/j.ajbls.20140206.15

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AMA Style

Lucia Winata Muslimin, Abdul Wahid Jamaluddin, Ressy Dwiyanti, Mochammad Hatta. Antimicrobial Inhibition on Zoonotic Bacterial Escherichia coli O157: H7 as a Cause of Food Borne Disease. Am J Biomed Life Sci. 2015;2(6):163-166. doi: 10.11648/j.ajbls.20140206.15

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@article{10.11648/j.ajbls.20140206.15,
  author = {Lucia Winata Muslimin and Abdul Wahid Jamaluddin and Ressy Dwiyanti and Mochammad Hatta},
  title = {Antimicrobial Inhibition on Zoonotic Bacterial Escherichia coli O157: H7 as a Cause of Food Borne Disease},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {2},
  number = {6},
  pages = {163-166},
  doi = {10.11648/j.ajbls.20140206.15},
  url = {https://doi.org/10.11648/j.ajbls.20140206.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20140206.15},
  abstract = {This study aims to accelerate zoonosis control system, secure food safety and improve the environmental quality. Meat samples, swab and water were acquired from five regions in South Sulawesi, Indonesia. The samples were implanted to bacterial growth medium inside the ice-box and carried to the lab to be inoculated with Nutrient Agar, Eosin Methylen Blue Agar Sorbitol Mac Conkey Agar (SMAC). IMVIC test, Biochemical Test and pathogenic test with blood Agar from the suspected Escherichia coli O157H7 result, was followed by PCR test to genetically identify the bacteria. The result was then examined for sensitivity test with antibiotics: Imipenem, Tetracycline, Erytromycin, Levofloxacine, Amoxycilin, Chloramphenicol and Ciprofloxacine. Among 117 samples. 43 was assumed positive with culture method, 12 was assumed positive with PCR E.coli O157H7.The most sensitive antibiotics, Imipenem, Ciprofloxacine and levofloxacine.},
 year = {2015}
}

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TY  - JOUR
T1  - Antimicrobial Inhibition on Zoonotic Bacterial Escherichia coli O157: H7 as a Cause of Food Borne Disease
AU  - Lucia Winata Muslimin
AU  - Abdul Wahid Jamaluddin
AU  - Ressy Dwiyanti
AU  - Mochammad Hatta
Y1  - 2015/01/08
PY  - 2015
N1  - https://doi.org/10.11648/j.ajbls.20140206.15
DO  - 10.11648/j.ajbls.20140206.15
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 163
EP  - 166
PB  - Science Publishing Group
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UR  - https://doi.org/10.11648/j.ajbls.20140206.15
AB  - This study aims to accelerate zoonosis control system, secure food safety and improve the environmental quality. Meat samples, swab and water were acquired from five regions in South Sulawesi, Indonesia. The samples were implanted to bacterial growth medium inside the ice-box and carried to the lab to be inoculated with Nutrient Agar, Eosin Methylen Blue Agar Sorbitol Mac Conkey Agar (SMAC). IMVIC test, Biochemical Test and pathogenic test with blood Agar from the suspected Escherichia coli O157H7 result, was followed by PCR test to genetically identify the bacteria. The result was then examined for sensitivity test with antibiotics: Imipenem, Tetracycline, Erytromycin, Levofloxacine, Amoxycilin, Chloramphenicol and Ciprofloxacine. Among 117 samples. 43 was assumed positive with culture method, 12 was assumed positive with PCR E.coli O157H7.The most sensitive antibiotics, Imipenem, Ciprofloxacine and levofloxacine.
VL  - 2
IS  - 6
ER  -

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Author Information

Lucia Winata Muslimin

Program study of Veterinary Medicine, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia
Abdul Wahid Jamaluddin

Program study of Veterinary Medicine, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia
Ressy Dwiyanti

Department Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia
Mochammad Hatta

Molecular Biology and Immunology Laboratoryfor Infectious Diseases, Department Microbiology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

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APA Style

Lucia Winata Muslimin, Abdul Wahid Jamaluddin, Ressy Dwiyanti, Mochammad Hatta. (2015). Antimicrobial Inhibition on Zoonotic Bacterial Escherichia coli O157: H7 as a Cause of Food Borne Disease. American Journal of Biomedical and Life Sciences, 2(6), 163-166. https://doi.org/10.11648/j.ajbls.20140206.15

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ACS Style

Lucia Winata Muslimin; Abdul Wahid Jamaluddin; Ressy Dwiyanti; Mochammad Hatta. Antimicrobial Inhibition on Zoonotic Bacterial Escherichia coli O157: H7 as a Cause of Food Borne Disease. Am. J. Biomed. Life Sci. 2015, 2(6), 163-166. doi: 10.11648/j.ajbls.20140206.15

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AMA Style

Lucia Winata Muslimin, Abdul Wahid Jamaluddin, Ressy Dwiyanti, Mochammad Hatta. Antimicrobial Inhibition on Zoonotic Bacterial Escherichia coli O157: H7 as a Cause of Food Borne Disease. Am J Biomed Life Sci. 2015;2(6):163-166. doi: 10.11648/j.ajbls.20140206.15

Copy | Download

@article{10.11648/j.ajbls.20140206.15,
  author = {Lucia Winata Muslimin and Abdul Wahid Jamaluddin and Ressy Dwiyanti and Mochammad Hatta},
  title = {Antimicrobial Inhibition on Zoonotic Bacterial Escherichia coli O157: H7 as a Cause of Food Borne Disease},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {2},
  number = {6},
  pages = {163-166},
  doi = {10.11648/j.ajbls.20140206.15},
  url = {https://doi.org/10.11648/j.ajbls.20140206.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20140206.15},
  abstract = {This study aims to accelerate zoonosis control system, secure food safety and improve the environmental quality. Meat samples, swab and water were acquired from five regions in South Sulawesi, Indonesia. The samples were implanted to bacterial growth medium inside the ice-box and carried to the lab to be inoculated with Nutrient Agar, Eosin Methylen Blue Agar Sorbitol Mac Conkey Agar (SMAC). IMVIC test, Biochemical Test and pathogenic test with blood Agar from the suspected Escherichia coli O157H7 result, was followed by PCR test to genetically identify the bacteria. The result was then examined for sensitivity test with antibiotics: Imipenem, Tetracycline, Erytromycin, Levofloxacine, Amoxycilin, Chloramphenicol and Ciprofloxacine. Among 117 samples. 43 was assumed positive with culture method, 12 was assumed positive with PCR E.coli O157H7.The most sensitive antibiotics, Imipenem, Ciprofloxacine and levofloxacine.},
 year = {2015}
}

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TY  - JOUR
T1  - Antimicrobial Inhibition on Zoonotic Bacterial Escherichia coli O157: H7 as a Cause of Food Borne Disease
AU  - Lucia Winata Muslimin
AU  - Abdul Wahid Jamaluddin
AU  - Ressy Dwiyanti
AU  - Mochammad Hatta
Y1  - 2015/01/08
PY  - 2015
N1  - https://doi.org/10.11648/j.ajbls.20140206.15
DO  - 10.11648/j.ajbls.20140206.15
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 163
EP  - 166
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20140206.15
AB  - This study aims to accelerate zoonosis control system, secure food safety and improve the environmental quality. Meat samples, swab and water were acquired from five regions in South Sulawesi, Indonesia. The samples were implanted to bacterial growth medium inside the ice-box and carried to the lab to be inoculated with Nutrient Agar, Eosin Methylen Blue Agar Sorbitol Mac Conkey Agar (SMAC). IMVIC test, Biochemical Test and pathogenic test with blood Agar from the suspected Escherichia coli O157H7 result, was followed by PCR test to genetically identify the bacteria. The result was then examined for sensitivity test with antibiotics: Imipenem, Tetracycline, Erytromycin, Levofloxacine, Amoxycilin, Chloramphenicol and Ciprofloxacine. Among 117 samples. 43 was assumed positive with culture method, 12 was assumed positive with PCR E.coli O157H7.The most sensitive antibiotics, Imipenem, Ciprofloxacine and levofloxacine.
VL  - 2
IS  - 6
ER  -

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