Homeobox genes are present in the genome of the honeybee Apis mellifera and their expression has been studied principally during embryonic development. The aim of this research was to evaluate expression of three key Hox genes from the larval period to the adult. The expression of honeybee GB51301, Ant and Ubx were examined in 24 and 72 hrs of larvae and pupa, classified on the basis of eye colours, using quantitative RT PCR on either the entire body or the head only. The results show that levels of expression from these genes change in relation to the development of various organs. GB51301(HOX3/zen) expression is mainly localised in the head, and it is expressed in the larvae, when the first nervous cells are formed and in the adult worker. ANTP is expressed in the white-eye pupa at a similar time to the development of antenna. UBX is also expressed in white and red eye pupae at a similar time to leg formation. The expression of these genes is practically absent in the brown eye pupa, when the development of these organs is completed. These genes are found mainly in the body.
| Published in |
American Journal of BioScience (Volume 2, Issue 6-1)
This article belongs to the Special Issue Chemical Biology |
| DOI | 10.11648/j.ajbio.s.2014020601.18 |
| Page(s) | 43-48 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
Apis mellifera Honey Bee, Development, Hox Genes, Expression
| [1] | Rembold H., Kremer J.P., Ulrich G.M. (1980) Characterization of postembryonic development al stages of the female castles of the honey bee, Apis mellifera Apidologie,11, 29-31. |
| [2] | E.R. de F Michelette, A.E.E. Soares (1993) Characterization of preimaginal developmental stages in Africanized honey bee workers (Apis Mellifera L), Apidologie, 24,431-440. |
| [3] | Bomtorin A.D., Barchuk,Robert A., Moda L.M. Simoes, P., Zila L. (2012) Hox gene expression leads to differential hind leg development between honey bee castes, PLoS ONE 7(7): Zheng A., Li J., Begna D., Fang Y., Feng M., Song F. (2011) Proteomic Analysis of Honeybee (Apis mellifera L.) pupae head development, PLoS ONE 6(5): e20428. doi:10.1371/journal.pone.0020428. |
| [4] | Waldorf U., Binner P., Fleig R. (2000) Hox genes in the honey bee Apis mellifera, Dev. Genes Evol.,210, 483-492 |
| [5] | Falciani F., Hausdort B. Schroder R.,Akam M.,Tautz D., Denell R., Brown S. (1996) Class 3 Hox genes in insects and the origin of zen, Proc. Nat. Acad. Sci.USA,93(16), 8479-8484. |
| [6] | Dearden P.K. , Wilson M.J., Sablan L.,Osborne P.W., Havler M. McNaughton E., Kimura K., Milshima N.V., Hasselman M., Gempe T., Schioett M., Brown S.J., Elsik C. G., Holland P. W. H., Kadowaki T., Beye M. (2006) Patterns of conservation and change in honey bee development genes, Genome research 16, 1376-1384. |
| [7] | Rushlow C., Levine M. (1990) Role of the zerknüllt gene in dorsal-ventral pattern formation in Drosophila. Adv. Genet.27,277-307. |
| [8] | Kaufman T.C., Lewis R., Wakimoto B.T. (1980) Cytogenetic analysis of chromosome 3 in Drosophila melanogaster, the homeotic gene complex in polytene chromosome interval 84A-B Genetics 94; 115-133. |
| [9] | Lewis B. (1978) A complex controlling segmentation in Drosophila Nature 276 565-570. |
| [10] | Warren R.W., Nagy L., Seleque J., Gates J., Carroll S.B. (1994) Evolution of homeotic gene regulation and function in flies and butterflies, Nature 372: 458-461. |
| [11] | Fleig R., Walldorf U., Gehring WJ., Sander K. (1988) In situ localization of the transcripts of a homeobox gene in the honey bee Apis mellifera L. (Hymenoptera) Rouxs Arch Dev Biol 201; 235-242. |
| [12] | Waldorf U., Fleig R., Gehring W.J. (1989) Comparison of homeobox-containing genes in the honeybee and Drosophila Proc Natl Acad Sci USA 86; 9971-9975. |
| [13] | Medved V. Huang ZY Popadic A. (2014) Ubx promotes corbicula development in Apis mellifera. Biol. Lett. 10:20131021. |
| [14] | Roat T.C., Landim C.D.C (2010) Mitosis and cell death in the optic lobes of workers, queens and drones of the honey bee (Apis mellifera) during metamorphosis, J. Biosci. 35, 415-425. |
| [15] | Livak J. Kenneth, Thomas D. Schmittgen (2001) Analysis of Relative Gene Expression Data using Real-Time Quantitative PCR and 2-ΔΔCT Method, Methods, 25, 402-408. |
| [16] | Shao R., Chen Q., Bao X.,Wu Q.,Chen Q., Yu Z. (1992) Correlation between the head weight and royal Jelly gland weight in Italian honeybee, Journal of Fujan Agricultural College 21,101-103. |
| [17] | Panov. A. (1957) The structure of the brain in insects in successive stages of postembryonic development; Ent. Obozr. 36, 269–284. |
| [18] | Farris S. M., Robinson G. E., Davis R. L., Fahrbach S. E.(1999) Larval and pupal development of the mushroom bodies in the honey bee, Apis mellifera; J. Comp. Neurol. 414 (1), 97–113. |
| [19] | Waldorf U., Binner P., Fleig R. (2000) Hox genes in the honey bee Apis mellifera, Dev. Genes Evol.,210, 483-492. |
APA Style
Mariapia Viola-Magni, Samuela Cataldi. (2014). Expression of Hox Genes, Zen (GB 51301) ANT and UBX during the Development of Honey Bee (Apis mellifera). American Journal of BioScience, 2(6-1), 43-48. https://doi.org/10.11648/j.ajbio.s.2014020601.18
ACS Style
Mariapia Viola-Magni; Samuela Cataldi. Expression of Hox Genes, Zen (GB 51301) ANT and UBX during the Development of Honey Bee (Apis mellifera). Am. J. BioScience 2014, 2(6-1), 43-48. doi: 10.11648/j.ajbio.s.2014020601.18
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.ajbio.s.2014020601.18,
author = {Mariapia Viola-Magni and Samuela Cataldi},
title = {Expression of Hox Genes, Zen (GB 51301) ANT and UBX during the Development of Honey Bee (Apis mellifera)},
journal = {American Journal of BioScience},
volume = {2},
number = {6-1},
pages = {43-48},
doi = {10.11648/j.ajbio.s.2014020601.18},
url = {https://doi.org/10.11648/j.ajbio.s.2014020601.18},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.s.2014020601.18},
abstract = {Homeobox genes are present in the genome of the honeybee Apis mellifera and their expression has been studied principally during embryonic development. The aim of this research was to evaluate expression of three key Hox genes from the larval period to the adult. The expression of honeybee GB51301, Ant and Ubx were examined in 24 and 72 hrs of larvae and pupa, classified on the basis of eye colours, using quantitative RT PCR on either the entire body or the head only. The results show that levels of expression from these genes change in relation to the development of various organs. GB51301(HOX3/zen) expression is mainly localised in the head, and it is expressed in the larvae, when the first nervous cells are formed and in the adult worker. ANTP is expressed in the white-eye pupa at a similar time to the development of antenna. UBX is also expressed in white and red eye pupae at a similar time to leg formation. The expression of these genes is practically absent in the brown eye pupa, when the development of these organs is completed. These genes are found mainly in the body.},
year = {2014}
}
TY - JOUR T1 - Expression of Hox Genes, Zen (GB 51301) ANT and UBX during the Development of Honey Bee (Apis mellifera) AU - Mariapia Viola-Magni AU - Samuela Cataldi Y1 - 2014/09/02 PY - 2014 N1 - https://doi.org/10.11648/j.ajbio.s.2014020601.18 DO - 10.11648/j.ajbio.s.2014020601.18 T2 - American Journal of BioScience JF - American Journal of BioScience JO - American Journal of BioScience SP - 43 EP - 48 PB - Science Publishing Group SN - 2330-0167 UR - https://doi.org/10.11648/j.ajbio.s.2014020601.18 AB - Homeobox genes are present in the genome of the honeybee Apis mellifera and their expression has been studied principally during embryonic development. The aim of this research was to evaluate expression of three key Hox genes from the larval period to the adult. The expression of honeybee GB51301, Ant and Ubx were examined in 24 and 72 hrs of larvae and pupa, classified on the basis of eye colours, using quantitative RT PCR on either the entire body or the head only. The results show that levels of expression from these genes change in relation to the development of various organs. GB51301(HOX3/zen) expression is mainly localised in the head, and it is expressed in the larvae, when the first nervous cells are formed and in the adult worker. ANTP is expressed in the white-eye pupa at a similar time to the development of antenna. UBX is also expressed in white and red eye pupae at a similar time to leg formation. The expression of these genes is practically absent in the brown eye pupa, when the development of these organs is completed. These genes are found mainly in the body. VL - 2 IS - 6-1 ER -
Email: