Probiotic Induce Macrophage Cytokine Production Via Activation of STAT-3 Pathway

Neama Y; Habil

doi:doi:10.11648/j.acis.s.2015030201.11

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Probiotic Induce Macrophage Cytokine Production Via Activation of STAT-3 Pathway

Neama Y, Habil

Published in Automation, Control and Intelligent Systems (Volume 3, Issue 2-1)

Received: 21 December 2014 Accepted: 25 December 2014 Published: 18 January 2015

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Abstract

Macrophages are mononuclear phagocytes generated from monocyte emigrated from blood circulation. Macrophages mediated the innate and adaptive immunity through different routes, and cytokine production is one of these routes. Signal transducer and activators of transcription (STATs) are cytoplasmic transcription factors that are key mediators of cytokine and growth factor signalling pathways. STAT-3 is implicated in macrophage cytokine signalling and production. It's well reported that the microbiota is very important as it primes the immune system for the antigens encountered later in life. Probiotics defined as ‘Live microorganisms which when administered in adequate amounts confer a health benefit on the host. Therefore, the aim of this project was to answer the question whether probiotics induced cytokine production via activation of STAT-3 signalling pathway.Results showed that probiotic Lactobacillus casie strain Shirota was successfully induced cytokine production via activation of STAT-3 by anti-inflammatory macrophages induced by TNF-α. The findings of this study will open new strategy to modulate the immune response by probiotic bacterialeading to treat the diseasesthat related with irregular cytokine production.

Published in	Automation, Control and Intelligent Systems (Volume 3, Issue 2-1) This article belongs to the Special Issue Artificial Nano Sensory System
DOI	10.11648/j.acis.s.2015030201.11
Page(s)	1-7
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Macrophages, Probiotics, Cytokines, STAT-3

References

[1]	AHMED, S. T. & IVASHKIV, L. B. 2000. Inhibition of IL-6 and IL-10 Signaling and Stat Activation by Inflammatory and Stress Pathways. The Journal of Immunology, 165, 5227-5237.
[2]	AKHTAR, M., WATSON, J. L., NAZLI, A. & MCKAY, D. M. 2003. Bacterial DNA evokes epithelial IL-8 production by a MAPK-dependent, NF-[kappa]B-independent pathway. FASEB J., 17, 1319-1321.
[3]	BIETHAHN, S., ALVES, F., WILDE, S., HIDDEMANN, W. & SPIEKERMANN, K. 1999. Expression of granulocyte colony-stimulating factor– and granulocyte-macrophage colony-stimulating factor–associated signal transduction proteins of the JAK/STAT pathway in normal granulopoiesis and in blast cells of acute myelogenous leukemia. Experimental hematology, 27, 885-894.
[4]	BROMBERG, J. 2002 Stat proteins and oncogenesis. J Clin Invest. :, 09(9), 1139-1142.
[5]	BUECHLER, C., RITTER, M., ORSÓ, E., LANGMANN, T., KLUCKEN, J. & SCHMITZ, G. 2000. Regulation of scavenger receptor CD163 expression in human monocytes and macrophages by pro- and antiinflammatory stimuli. Journal of Leukocyte Biology, 67, 97-103.
[6]	CHENG, F., WANG, H.-W., CUENCA, A., HUANG, M., GHANSAH, T., BRAYER, J., KERR, W. G., TAKEDA, K., AKIRA, S., SCHOENBERGER, S. P., YU, H., JOVE, R. & SOTOMAYOR, E. M. 2003. A Critical Role for Stat3 Signaling in Immune Tolerance. Immunity, 19, 425-436.
[7]	DARNELL, J., KERR, I. & STARK, G. 1994. Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins. Science, 264, 1415-1421.
[8]	DONNELLY, R., DICKENSHEETS, H. & FINBLOOM, D. 1999. The interleukin-10 signal transduction pathway and regulation of gene expression in mononuclear phagocytes. J Interferon Cytokine Res, 19(6), 563-73.
[9]	DOTAN, I. & RACHMILEWITZ, D. 2005. Probiotics in inflammatory bowel disease: possible mechanisms of action. Current Opinion in Gastroenterology, 21, 426-430.
[10]	DUFFIELD, J. S. 2003. The inflammatory macrophage: a story of Jekyll and Hyde. Clin. Sci., 104, 27-38.
[11]	FOEY, A. 2012. Mucosal macrophages: phenotype and functionality in homeostasis and pathology. In. Handbook of macrophages: life cycle, functions and diseases. Eds. Takahashi, R. and Kai, H. Nova Science Publishers Inc., NY, USA. Invited review chapter.
[12]	FUKADA, T., HIBI, M., YAMANAKA, Y., TAKAHASHI-TEZUKA, M., FUJITANI, Y., YAMAGUCHI, T., NAKAJIMA, K. & HIRANO, T. 1996. Two Signals Are Necessary for Cell Proliferation Induced by a Cytokine Receptor gp130: Involvement of STAT3 in Anti-Apoptosis. Immunity, 5, 449-460.
[13]	GALDEANO, C. M. & PERDIGÓN, G. 2006. The Probiotic Bacterium Lactobacillus casei Induces Activation of the Gut Mucosal Immune System through Innate Immunity. Clinical and Vaccine Immunology, 13, 219-226.
[14]	GORDON, S. 2003. Alternative activation of macrophages. Nat Rev Immunol, 3, 23-35.
[15]	GRIVENNIKOV, S., KARIN, E., TERZIC, J., MUCIDA, D., YU, G., VALLABHAPURAPU, S., SCHELLER, J., ROSE-JOHN, S., CHEROUTRE, H., ECKMANN, L. & KARIN, M. 2009. IL-6 and STAT3 are required for survival of intestinal epithelial cells and development of colitis associated cancer. Cancer Cell, 3; 15(2), 103-113.
[16]	HABIL, N., ABATE, W., BEAL, J. & FOEY, A. D. 2014. Heat-killed probiotic bacteria differentially regulate colonic epithelial cell production of human β-defensin-2: dependence on inflammatory cytokines. Beneficial Microbes, 5, 483-495.
[17]	HABIL, N., BEAL, J. & FOEY, A. 2011. Probiotic bacterial strains differentially modulate macrophage cytokine in a strain-depent and cell subset-specific manner. Beneficial Microbe, 2(4), 283-293.
[18]	HABIL, N., BEAL, J. & FOEY, A. 2012. Lactobacillus casei strain Shirota selectively modulates macrophage subset cytokine production. Int. J. Probiotics & Prebiotics 7(1), 1-12.
[19]	HABIL, N., FOEY, A. & BEAL, J. 2013. Probiotic Modulation of Mucosal Immune Responses in an In Vitro Co-Culture Model.
[20]	HALLER, D., BODE, C., HAMMES, W. P., PFEIFER, A. M. A., SCHIFFRIN, E. J. & BLUM, S. 2000. Non-pathogenic bacteria elicit a differential cytokine response by intestinal epithelial cell/leucocyte co-cultures. Gut, 47, 79-87.
[21]	HARRINGTON, L. E., HATTON, R. D., MANGAN, P. R., TURNER, H., MURPHY, T. L., MURPHY, K. M. & WEAVER, C. T. 2005. Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages. Nat Immunol, 6, 1123-1132.
[22]	HUME, D. A. 2006. The mononuclear phagocyte system. Current Opinion in Immunology, 18, 49-53.
[23]	KIM, S. O., SHEIKH, H. I., HA, S.-D., MARTINS, A. & REID, G. 2006. G-CSF-mediated inhibition of JNK is a key mechanism for Lactobacillus rhamnosus-induced suppression of TNF production in macrophages. Cellular Microbiology, 8, 1958-1971.
[24]	KOVALOVICH, K., LI, W., DEANGELIS, R., GREENBAUM, L. E., CILIBERTO, G. & TAUB, R. 2001. Interleukin-6 Protects against Fas-mediated Death by Establishing a Critical Level of Anti-apoptotic Hepatic Proteins FLIP, Bcl-2, and Bcl-xL. Journal of Biological Chemistry, 276, 26605-26613.
[25]	MADSEN, K., CORNISH, A., SOPER, P., MCKAIGNEY, C., JIJON, H., YACHIMEC, C., DOYLE, J., JEWELL, L. & DE SIMONE, C. 2001. Probiotic Bacteria Enhance Murine and Human Intestinal Epithelial Barrier Function. Gastroenterology, 121, 580-591.
[26]	MANTOVANI, A., SICA, A. & LOCATI, M. 2007. New vistas on macrophage differentiation and activation. European Journal of Immunology, 37, 14-16.
[27]	MATSUGUCHI, T., TAKAGI, A., MATSUZAKI, T., NAGAOKA, M., ISHIKAWA, K., YOKOKURA, T. & YOSHIKAI, Y. 2003. Lipoteichoic Acids from Lactobacillus Strains Elicit Strong Tumor Necrosis Factor Alpha-Inducing Activities in Macrophages through Toll-Like Receptor 2. Clinical and Diagnostic Laboratory Immunology, 10, 259-266.
[28]	MATSUKAWA, A., KUDO, S., MAEDA, T., NUMATA, K., WATANABE, H., TAKEDA, K., AKIRA, S. & ITO, T. 2005. Stat3 in Resident Macrophages as a Repressor Protein of Inflammatory Response. The Journal of Immunology, 175, 3354-3359.
[29]	MATSUMOTO, S., HARA, T., HORI, T., MITSUYAMA, K., NAGAOKA, M., TOMIYASU, N., SUZUKI, A. & SATA, M. 2005. Probiotic Lactobacillus-induced improvement in murine chronic inflammatory bowel disease is associated with the down-regulation of pro-inflammatory cytokines in lamina propria mononuclear cells. Clinical & Experimental Immunology, 140, 417-426.
[30]	MATSUMOTO, S., HARA, T., MITSUYAMA, K., YAMAMOTO, M., TSURUTA, O., SATA, M., SCHELLER, J., ROSE-JOHN, S., KADO, S.-I. & TAKADA, T. 2010. Essential Roles of IL-6 Trans-Signaling in Colonic Epithelial Cells, Induced by the IL-6/Soluble IL-6 Receptor Derived from Lamina Propria Macrophages, on the Development of Colitis-Associated Premalignant Cancer in a Murine Model. The Journal of Immunology, 184, 1543-1551.
[31]	MATSUMOTO, S., HARA, T., NAGAOKA, M., MIKE, A., MITSUYAMA, K., SAKO, T., YAMAMOTO, M., KADO, S. & TAKADA, T. 2009. A component of polysaccharide peptidoglycan complex on Lactobacillus induced an improvement of murine model of inflammatory bowel disease and colitis-associated cancer. Immunology, 128, e170-e180.
[32]	MOSSER, D. M. & EDWARDS, J. P. 2008. Exploring the full spectrum of macrophage activation. Nat Rev Immunol, 8, 958-969.
[33]	O'ROURKE, L. & SHEPHERD, P. R. 2002. Biphasic regulation of extracellular-signal-regulated protein kinase by leptin in macrophages: role in regulating STAT3 Ser727 phosphorylation and DNA binding. Biochem. J., 364, 875-879.
[34]	PAOLILLO, R., ROMANO CARRATELLI, C., SORRENTINO, S., MAZZOLA, N. & RIZZO, A. 2009. Immunomodulatory effects of Lactobacillus plantarum on human colon cancer cells. International Immunopharmacology, 9, 1265-1271.
[35]	SAVVIDOU, S. 2009. Selection of a chicken Lactobacillus strain with probiotic properties and its application in poultry production. . PhD thesis, University of Plymouth, Plymouth, UK.
[36]	SHIDA, K., NANNO, M. & NAGATA, S. 2011. Flexible cytokine production by macrophages and T cells in response to probiotic bacteria: A possible mechanism by which probiotics exert multifunctional immune regulatory activities. Gut Microbes, 2, 109-114.
[37]	STEIDLER, L., HANS, W., SCHOTTE, L., NEIRYNCK, S., OBERMEIER, F., FALK, W., FIERS, W. & REMAUT, E. 2000. Treatment of Murine Colitis by Lactococcus lactis Secreting Interleukin-10. Science, 289, 1352-1355.
[38]	TAKEDA, K., CLAUSEN, B. E., KAISHO, T., TSUJIMURA, T., TERADA, N., FÖRSTER, I. & AKIRA, S. 1999. Enhanced Th1 Activity and Development of Chronic Enterocolitis in Mice Devoid of Stat3 in Macrophages and Neutrophils. Immunity, 10, 39-49.
[39]	VERRECK, F. A. W., DE BOER, T., LANGENBERG, D. M. L., HOEVE, M. A., KRAMER, M., VAISBERG, E., KASTELEIN, R., KOLK, A., DE WAAL-MALEFYT, R. & OTTENHOFF, T. H. M. 2004. Human IL-23-producing type 1 macrophages promote but IL-10-producing type 2 macrophages subvert immunity to (myco)bacteria. Proceedings of the National Academy of Sciences of the United States of America, 101, 4560-4565.
[40]	WILLIAMS, L., BRADLEY, L., SMITH, A. & FOXWELL, B. 2004. Signal Transducer and Activator of Transcription 3 Is the Dominant Mediator of the Anti-Inflammatory Effects of IL-10 in Human Macrophages. The Journal of Immunology, 172, 567-576.
[41]	WILLIAMS, L. M., SARMA, U., WILLETS, K., SMALLIE, T., BRENNAN, F. & FOXWELL, B. M. J. 2007. Expression of Constitutively Active STAT3 Can Replicate the Cytokine-suppressive Activity of Interleukin-10 in Human Primary Macrophages. Journal of Biological Chemistry, 282, 6965-6975.
[42]	WINKLER, P., GHADIMI, D., SCHREZENMEIR, J. & KRAEHENBUHL, J.-P. 2007. Molecular and Cellular Basis of Microflora-Host Interactions. J. Nutr., 137, 756S-772.
[43]	YANG, J., LIAO, X., AGARWAL, M. K., BARNES, L., AURON, P. E. & STARK, G. R. 2007. Unphosphorylated STAT3 accumulates in response to IL-6 and activates transcription by binding to NFkappaB. Genes & development, 21, 1396-1408.

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Neama Y, Habil. (2015). Probiotic Induce Macrophage Cytokine Production Via Activation of STAT-3 Pathway. Automation, Control and Intelligent Systems, 3(2-1), 1-7. https://doi.org/10.11648/j.acis.s.2015030201.11

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Neama Y; Habil. Probiotic Induce Macrophage Cytokine Production Via Activation of STAT-3 Pathway. Autom. Control Intell. Syst. 2015, 3(2-1), 1-7. doi: 10.11648/j.acis.s.2015030201.11

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AMA Style

Neama Y, Habil. Probiotic Induce Macrophage Cytokine Production Via Activation of STAT-3 Pathway. Autom Control Intell Syst. 2015;3(2-1):1-7. doi: 10.11648/j.acis.s.2015030201.11

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@article{10.11648/j.acis.s.2015030201.11,
  author = {Neama Y and Habil},
  title = {Probiotic Induce Macrophage Cytokine Production Via Activation of STAT-3 Pathway},
  journal = {Automation, Control and Intelligent Systems},
  volume = {3},
  number = {2-1},
  pages = {1-7},
  doi = {10.11648/j.acis.s.2015030201.11},
  url = {https://doi.org/10.11648/j.acis.s.2015030201.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.acis.s.2015030201.11},
  abstract = {Macrophages are mononuclear phagocytes generated from monocyte emigrated from blood circulation. Macrophages mediated the innate and adaptive immunity through different routes, and cytokine production is one of these routes. Signal transducer and activators of transcription (STATs) are cytoplasmic transcription factors that are key mediators of cytokine and growth factor signalling pathways. STAT-3 is implicated in macrophage cytokine signalling and production. It's well reported that the microbiota is very important as it primes the immune system for the antigens encountered later in life. Probiotics defined as ‘Live microorganisms which when administered in adequate amounts confer a health benefit on the host. Therefore, the aim of this project was to answer the question whether probiotics induced cytokine production via activation of STAT-3 signalling pathway.Results showed that probiotic Lactobacillus casie strain Shirota was successfully induced cytokine production via activation of STAT-3 by anti-inflammatory macrophages induced by TNF-α. The findings of this study will open new strategy to modulate the immune response by probiotic bacterialeading to treat the diseasesthat related with irregular cytokine production.},
 year = {2015}
}

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TY  - JOUR
T1  - Probiotic Induce Macrophage Cytokine Production Via Activation of STAT-3 Pathway
AU  - Neama Y
AU  - Habil
Y1  - 2015/01/18
PY  - 2015
N1  - https://doi.org/10.11648/j.acis.s.2015030201.11
DO  - 10.11648/j.acis.s.2015030201.11
T2  - Automation, Control and Intelligent Systems
JF  - Automation, Control and Intelligent Systems
JO  - Automation, Control and Intelligent Systems
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2328-5591
UR  - https://doi.org/10.11648/j.acis.s.2015030201.11
AB  - Macrophages are mononuclear phagocytes generated from monocyte emigrated from blood circulation. Macrophages mediated the innate and adaptive immunity through different routes, and cytokine production is one of these routes. Signal transducer and activators of transcription (STATs) are cytoplasmic transcription factors that are key mediators of cytokine and growth factor signalling pathways. STAT-3 is implicated in macrophage cytokine signalling and production. It's well reported that the microbiota is very important as it primes the immune system for the antigens encountered later in life. Probiotics defined as ‘Live microorganisms which when administered in adequate amounts confer a health benefit on the host. Therefore, the aim of this project was to answer the question whether probiotics induced cytokine production via activation of STAT-3 signalling pathway.Results showed that probiotic Lactobacillus casie strain Shirota was successfully induced cytokine production via activation of STAT-3 by anti-inflammatory macrophages induced by TNF-α. The findings of this study will open new strategy to modulate the immune response by probiotic bacterialeading to treat the diseasesthat related with irregular cytokine production.
VL  - 3
IS  - 2-1
ER  -

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Neama Y
Habil

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APA Style

Neama Y, Habil. (2015). Probiotic Induce Macrophage Cytokine Production Via Activation of STAT-3 Pathway. Automation, Control and Intelligent Systems, 3(2-1), 1-7. https://doi.org/10.11648/j.acis.s.2015030201.11

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ACS Style

Neama Y; Habil. Probiotic Induce Macrophage Cytokine Production Via Activation of STAT-3 Pathway. Autom. Control Intell. Syst. 2015, 3(2-1), 1-7. doi: 10.11648/j.acis.s.2015030201.11

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AMA Style

Neama Y, Habil. Probiotic Induce Macrophage Cytokine Production Via Activation of STAT-3 Pathway. Autom Control Intell Syst. 2015;3(2-1):1-7. doi: 10.11648/j.acis.s.2015030201.11

Copy | Download

@article{10.11648/j.acis.s.2015030201.11,
  author = {Neama Y and Habil},
  title = {Probiotic Induce Macrophage Cytokine Production Via Activation of STAT-3 Pathway},
  journal = {Automation, Control and Intelligent Systems},
  volume = {3},
  number = {2-1},
  pages = {1-7},
  doi = {10.11648/j.acis.s.2015030201.11},
  url = {https://doi.org/10.11648/j.acis.s.2015030201.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.acis.s.2015030201.11},
  abstract = {Macrophages are mononuclear phagocytes generated from monocyte emigrated from blood circulation. Macrophages mediated the innate and adaptive immunity through different routes, and cytokine production is one of these routes. Signal transducer and activators of transcription (STATs) are cytoplasmic transcription factors that are key mediators of cytokine and growth factor signalling pathways. STAT-3 is implicated in macrophage cytokine signalling and production. It's well reported that the microbiota is very important as it primes the immune system for the antigens encountered later in life. Probiotics defined as ‘Live microorganisms which when administered in adequate amounts confer a health benefit on the host. Therefore, the aim of this project was to answer the question whether probiotics induced cytokine production via activation of STAT-3 signalling pathway.Results showed that probiotic Lactobacillus casie strain Shirota was successfully induced cytokine production via activation of STAT-3 by anti-inflammatory macrophages induced by TNF-α. The findings of this study will open new strategy to modulate the immune response by probiotic bacterialeading to treat the diseasesthat related with irregular cytokine production.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Probiotic Induce Macrophage Cytokine Production Via Activation of STAT-3 Pathway
AU  - Neama Y
AU  - Habil
Y1  - 2015/01/18
PY  - 2015
N1  - https://doi.org/10.11648/j.acis.s.2015030201.11
DO  - 10.11648/j.acis.s.2015030201.11
T2  - Automation, Control and Intelligent Systems
JF  - Automation, Control and Intelligent Systems
JO  - Automation, Control and Intelligent Systems
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2328-5591
UR  - https://doi.org/10.11648/j.acis.s.2015030201.11
AB  - Macrophages are mononuclear phagocytes generated from monocyte emigrated from blood circulation. Macrophages mediated the innate and adaptive immunity through different routes, and cytokine production is one of these routes. Signal transducer and activators of transcription (STATs) are cytoplasmic transcription factors that are key mediators of cytokine and growth factor signalling pathways. STAT-3 is implicated in macrophage cytokine signalling and production. It's well reported that the microbiota is very important as it primes the immune system for the antigens encountered later in life. Probiotics defined as ‘Live microorganisms which when administered in adequate amounts confer a health benefit on the host. Therefore, the aim of this project was to answer the question whether probiotics induced cytokine production via activation of STAT-3 signalling pathway.Results showed that probiotic Lactobacillus casie strain Shirota was successfully induced cytokine production via activation of STAT-3 by anti-inflammatory macrophages induced by TNF-α. The findings of this study will open new strategy to modulate the immune response by probiotic bacterialeading to treat the diseasesthat related with irregular cytokine production.
VL  - 3
IS  - 2-1
ER  -

Copy | Download