Enzymatic Biosensor Associated with Molecularly Imprinted Polymers for Sensitive and Selective Detection of Organophosphorus Insecticides in Olive Oil

Ben Oujji Najwa; Bakas Idriss; Istamboulie Georges; Ait-Ichou Ihya; Ait-Addi Elhabib; Rouillon Régis; Noguer Thierry

doi:doi:10.11648/j.si.s.2014020601.11

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Enzymatic Biosensor Associated with Molecularly Imprinted Polymers for Sensitive and Selective Detection of Organophosphorus Insecticides in Olive Oil

Ben Oujji Najwa, Bakas Idriss, Istamboulie Georges, Ait-Ichou Ihya, Ait-Addi Elhabib, Rouillon Régis, Noguer Thierry

Published in Science Innovation (Volume 2, Issue 6-1)

Received: 13 September 2014 Accepted: 17 September 2014 Published: 15 October 2014

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Abstract

5 different bio-tools have been developed for sensitive detection of three organophosphorus insecticides mainly used for the treatment of olive trees: Malaoxon, Omethoate and Methidathion-oxon. The systems are based on the immobilization of electric eel acetylcholinesterase on screen-printed electrode (SPE) and microtiter plates by 3 different methods of immobilization. These developed systems were optimized with laboratory samples and then tested on natural samples of olive oil after a simple liquid-liquid extraction. Among these five systems designed, the amperometric biosensor based on acetylcholinesterase immobilized by bioencapsulation in a sol-gel matrix presented the best performance in terms of operational stability, storage stability, reproducibility, and it proved to be best suited for the determination of insecticides in real samples of olive oil. This biosensor has been associated with a highly selective extraction method based on the use of Molecularly Imprinted Polymers (MIPs) to improve selectivity. The results showed that the association MIPs-biosensor can selectively detect and quantify the three organophosphorus insecticides in such a complex matrix as olive oil, with limits of detection much lower than the maximum residue limit tolerated by international regulations.

Published in	Science Innovation (Volume 2, Issue 6-1) This article belongs to the Special Issue Biosensors: Innovative Technique for Food Safety and Environmental Monitoring
DOI	10.11648/j.si.s.2014020601.11
Page(s)	1-6
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Organophosphorus Insecticides, Electric Eel Acetylcholinesterase, Molecularly Imprinted Polymer (MIP), Biosensors, Olive Oil

References

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[3]	Lentza-Rizos, C., Avramides, E.J. Pesticide residues in olive oil. Arch. Environ. Contam. Toxicol. 141, 1995, 111-134.
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[11]	Ben Oujji, N., Bakas, I., Istamboulié, G., Ait-Ichou, I., Ait-Addi, E., Rouillon, R., Noguer, T. Sol-gel immobilization of acetylcholinesterase for the determination of organophosphate pesticides in olive oil with biosensors. Food Control, 30, 2013, 657-661.
[12]	Istamboulie, G., Fournier, D., Marty, J-L., Noguer, T. Phosphotriesterase: A complementary tool for the selective detection of two organophosphate insecticides: Chlorpyrifos and chlorfenvinfos. Talanta. 77, 2009, 1627–1631.
[13]	Rhouati, A., Istamboulie, G., Cortina-Puig, M., Marty, J-L., Noguer, T. Selective spectrophotometric detection of insecticides using cholinesterases, phosphotriesterase and chemometric analysis. Enzyme. Microb. Tech., 46, 2010, 212–216.
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Ben Oujji Najwa, Bakas Idriss, Istamboulie Georges, Ait-Ichou Ihya, Ait-Addi Elhabib, et al. (2014). Enzymatic Biosensor Associated with Molecularly Imprinted Polymers for Sensitive and Selective Detection of Organophosphorus Insecticides in Olive Oil. Science Innovation, 2(6-1), 1-6. https://doi.org/10.11648/j.si.s.2014020601.11

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ACS Style

Ben Oujji Najwa; Bakas Idriss; Istamboulie Georges; Ait-Ichou Ihya; Ait-Addi Elhabib, et al. Enzymatic Biosensor Associated with Molecularly Imprinted Polymers for Sensitive and Selective Detection of Organophosphorus Insecticides in Olive Oil. Sci. Innov. 2014, 2(6-1), 1-6. doi: 10.11648/j.si.s.2014020601.11

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AMA Style

Ben Oujji Najwa, Bakas Idriss, Istamboulie Georges, Ait-Ichou Ihya, Ait-Addi Elhabib, et al. Enzymatic Biosensor Associated with Molecularly Imprinted Polymers for Sensitive and Selective Detection of Organophosphorus Insecticides in Olive Oil. Sci Innov. 2014;2(6-1):1-6. doi: 10.11648/j.si.s.2014020601.11

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@article{10.11648/j.si.s.2014020601.11,
  author = {Ben Oujji Najwa and Bakas Idriss and Istamboulie Georges and Ait-Ichou Ihya and Ait-Addi Elhabib and Rouillon Régis and Noguer Thierry},
  title = {Enzymatic Biosensor Associated with Molecularly Imprinted Polymers for Sensitive and Selective Detection of Organophosphorus Insecticides in Olive Oil},
  journal = {Science Innovation},
  volume = {2},
  number = {6-1},
  pages = {1-6},
  doi = {10.11648/j.si.s.2014020601.11},
  url = {https://doi.org/10.11648/j.si.s.2014020601.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.si.s.2014020601.11},
  abstract = {5 different bio-tools have been developed for sensitive detection of three organophosphorus insecticides mainly used for the treatment of olive trees: Malaoxon, Omethoate and Methidathion-oxon. The systems are based on the immobilization of electric eel acetylcholinesterase on screen-printed electrode (SPE) and microtiter plates by 3 different methods of immobilization. These developed systems were optimized with laboratory samples and then tested on natural samples of olive oil after a simple liquid-liquid extraction. Among these five systems designed, the amperometric biosensor based on acetylcholinesterase immobilized by bioencapsulation in a sol-gel matrix presented the best performance in terms of operational stability, storage stability, reproducibility, and it proved to be best suited for the determination of insecticides in real samples of olive oil. This biosensor has been associated with a highly selective extraction method based on the use of Molecularly Imprinted Polymers (MIPs) to improve selectivity. The results showed that the association MIPs-biosensor can selectively detect and quantify the three organophosphorus insecticides in such a complex matrix as olive oil, with limits of detection much lower than the maximum residue limit tolerated by international regulations.},
 year = {2014}
}

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TY  - JOUR
T1  - Enzymatic Biosensor Associated with Molecularly Imprinted Polymers for Sensitive and Selective Detection of Organophosphorus Insecticides in Olive Oil
AU  - Ben Oujji Najwa
AU  - Bakas Idriss
AU  - Istamboulie Georges
AU  - Ait-Ichou Ihya
AU  - Ait-Addi Elhabib
AU  - Rouillon Régis
AU  - Noguer Thierry
Y1  - 2014/10/15
PY  - 2014
N1  - https://doi.org/10.11648/j.si.s.2014020601.11
DO  - 10.11648/j.si.s.2014020601.11
T2  - Science Innovation
JF  - Science Innovation
JO  - Science Innovation
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2328-787X
UR  - https://doi.org/10.11648/j.si.s.2014020601.11
AB  - 5 different bio-tools have been developed for sensitive detection of three organophosphorus insecticides mainly used for the treatment of olive trees: Malaoxon, Omethoate and Methidathion-oxon. The systems are based on the immobilization of electric eel acetylcholinesterase on screen-printed electrode (SPE) and microtiter plates by 3 different methods of immobilization. These developed systems were optimized with laboratory samples and then tested on natural samples of olive oil after a simple liquid-liquid extraction. Among these five systems designed, the amperometric biosensor based on acetylcholinesterase immobilized by bioencapsulation in a sol-gel matrix presented the best performance in terms of operational stability, storage stability, reproducibility, and it proved to be best suited for the determination of insecticides in real samples of olive oil. This biosensor has been associated with a highly selective extraction method based on the use of Molecularly Imprinted Polymers (MIPs) to improve selectivity. The results showed that the association MIPs-biosensor can selectively detect and quantify the three organophosphorus insecticides in such a complex matrix as olive oil, with limits of detection much lower than the maximum residue limit tolerated by international regulations.
VL  - 2
IS  - 6-1
ER  -

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Author Information

Ben Oujji Najwa

University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France
Bakas Idriss

University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France
Istamboulie Georges

University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France
Ait-Ichou Ihya

University Ibn Zohr, AQUAMAR, Photocatalysis and Environment Team, Chemistry Department/Faculty of Science, BP 8106 Citè Dakhla, Agadir, Morocco
Ait-Addi Elhabib

University Ibn Zohr, AQUAMAR, Photocatalysis and Environment Team, Chemistry Department/Faculty of Science, BP 8106 Citè Dakhla, Agadir, Morocco
Rouillon Régis

University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France
Noguer Thierry

University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France

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APA Style

Ben Oujji Najwa, Bakas Idriss, Istamboulie Georges, Ait-Ichou Ihya, Ait-Addi Elhabib, et al. (2014). Enzymatic Biosensor Associated with Molecularly Imprinted Polymers for Sensitive and Selective Detection of Organophosphorus Insecticides in Olive Oil. Science Innovation, 2(6-1), 1-6. https://doi.org/10.11648/j.si.s.2014020601.11

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ACS Style

Ben Oujji Najwa; Bakas Idriss; Istamboulie Georges; Ait-Ichou Ihya; Ait-Addi Elhabib, et al. Enzymatic Biosensor Associated with Molecularly Imprinted Polymers for Sensitive and Selective Detection of Organophosphorus Insecticides in Olive Oil. Sci. Innov. 2014, 2(6-1), 1-6. doi: 10.11648/j.si.s.2014020601.11

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AMA Style

Ben Oujji Najwa, Bakas Idriss, Istamboulie Georges, Ait-Ichou Ihya, Ait-Addi Elhabib, et al. Enzymatic Biosensor Associated with Molecularly Imprinted Polymers for Sensitive and Selective Detection of Organophosphorus Insecticides in Olive Oil. Sci Innov. 2014;2(6-1):1-6. doi: 10.11648/j.si.s.2014020601.11

Copy | Download

@article{10.11648/j.si.s.2014020601.11,
  author = {Ben Oujji Najwa and Bakas Idriss and Istamboulie Georges and Ait-Ichou Ihya and Ait-Addi Elhabib and Rouillon Régis and Noguer Thierry},
  title = {Enzymatic Biosensor Associated with Molecularly Imprinted Polymers for Sensitive and Selective Detection of Organophosphorus Insecticides in Olive Oil},
  journal = {Science Innovation},
  volume = {2},
  number = {6-1},
  pages = {1-6},
  doi = {10.11648/j.si.s.2014020601.11},
  url = {https://doi.org/10.11648/j.si.s.2014020601.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.si.s.2014020601.11},
  abstract = {5 different bio-tools have been developed for sensitive detection of three organophosphorus insecticides mainly used for the treatment of olive trees: Malaoxon, Omethoate and Methidathion-oxon. The systems are based on the immobilization of electric eel acetylcholinesterase on screen-printed electrode (SPE) and microtiter plates by 3 different methods of immobilization. These developed systems were optimized with laboratory samples and then tested on natural samples of olive oil after a simple liquid-liquid extraction. Among these five systems designed, the amperometric biosensor based on acetylcholinesterase immobilized by bioencapsulation in a sol-gel matrix presented the best performance in terms of operational stability, storage stability, reproducibility, and it proved to be best suited for the determination of insecticides in real samples of olive oil. This biosensor has been associated with a highly selective extraction method based on the use of Molecularly Imprinted Polymers (MIPs) to improve selectivity. The results showed that the association MIPs-biosensor can selectively detect and quantify the three organophosphorus insecticides in such a complex matrix as olive oil, with limits of detection much lower than the maximum residue limit tolerated by international regulations.},
 year = {2014}
}

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TY  - JOUR
T1  - Enzymatic Biosensor Associated with Molecularly Imprinted Polymers for Sensitive and Selective Detection of Organophosphorus Insecticides in Olive Oil
AU  - Ben Oujji Najwa
AU  - Bakas Idriss
AU  - Istamboulie Georges
AU  - Ait-Ichou Ihya
AU  - Ait-Addi Elhabib
AU  - Rouillon Régis
AU  - Noguer Thierry
Y1  - 2014/10/15
PY  - 2014
N1  - https://doi.org/10.11648/j.si.s.2014020601.11
DO  - 10.11648/j.si.s.2014020601.11
T2  - Science Innovation
JF  - Science Innovation
JO  - Science Innovation
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2328-787X
UR  - https://doi.org/10.11648/j.si.s.2014020601.11
AB  - 5 different bio-tools have been developed for sensitive detection of three organophosphorus insecticides mainly used for the treatment of olive trees: Malaoxon, Omethoate and Methidathion-oxon. The systems are based on the immobilization of electric eel acetylcholinesterase on screen-printed electrode (SPE) and microtiter plates by 3 different methods of immobilization. These developed systems were optimized with laboratory samples and then tested on natural samples of olive oil after a simple liquid-liquid extraction. Among these five systems designed, the amperometric biosensor based on acetylcholinesterase immobilized by bioencapsulation in a sol-gel matrix presented the best performance in terms of operational stability, storage stability, reproducibility, and it proved to be best suited for the determination of insecticides in real samples of olive oil. This biosensor has been associated with a highly selective extraction method based on the use of Molecularly Imprinted Polymers (MIPs) to improve selectivity. The results showed that the association MIPs-biosensor can selectively detect and quantify the three organophosphorus insecticides in such a complex matrix as olive oil, with limits of detection much lower than the maximum residue limit tolerated by international regulations.
VL  - 2
IS  - 6-1
ER  -

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