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Effect of Essential Oils of Artemisia arborescens on Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa

Received: 31 July 2014     Accepted: 16 January 2015     Published: 14 March 2015
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Abstract

This study was carried out to investigate the potential use of Artemisia arborescens as a source of antimicrobial agents against pathogens. Essential oils of A. arborescens were collected by hydrodistillation. The antibacterial properties of A. arborescens essential oil was studied on the standard gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and Staphylococcus aureus (gram-positive bacteria), then agar disk diffusion, minimal inhibition concentration (MIC) and minimum bactericidal concentration (MBC) were detected. The results of agar disk diffusion tests showed the inhibition zones as follow: S. aureus 00-18 mm, E. coli 00-16 mm and 08-14 mm for P. aeruginosa. However, their antibacterial activities were lower than those of Gentamicin. The MIC for S. aureus and P. aeruginosa was between 33 and 66 mg/ml, and for Gram-negative bacteria of E. coli was between 66 and 132 mg/ml, while the MBC values of this oil against the tested bacterial strains were between 132 and 264 mg/ml.

Published in Journal of Plant Sciences (Volume 2, Issue 6-1)

This article belongs to the Special Issue Pharmacological and Biological Investigation of Medicinal Plants

DOI 10.11648/j.jps.s.2014020601.11
Page(s) 1-4
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Artemisia arborescens Essential Oil, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Antimicrobial Activity

References
[1] K.H.C. Baser and F. Demirci, Essential oils, Kirk-Othmer Encyclopedia of chemical Technology, 4th ed., Sussex: Wiley, 2011.
[2] K. Bremer and C.J. Humphries,“Generic monograph of the Asteraceae-Anthemideae. Bull. Brit. Mus. Lond. Bot,” 1993, 23, p. 71–177.
[3] K. Kubitzki, The families and genera of vascular plants. Vol VIII Flowering plants. Eudicots. Asterales. J.W. Kadereit and C. Jeffrey (Eds.), Springer-Verlag: Berlin Heidelberg, 2007, p.358.
[4] Haider SZ, Mohan M and Andola HC. “Constituents of Artemisia indica Willd. from Uttarakhand Himalaya: A source of davanone,” Phcog Res 2014; 6: 257-9.
[5] J. Yashphe, I. Feuerstein, S. Barel and R. Segal, “The antibacterial and antispasmodic activity of Artemisia herba-alba Asso. II. Examination of essential oils from various chemotypes,” Int. J. Crude. Drug Res, 1987, 25, p. 89–96.
[6] N. Li, Y. Mao, C. Deng and X. Zhang, “Separation and identification of volatile constituents in Artemisia argyi flowers by GC-MS with SPME and steam distillation,” J Chromatogr Sci, 2008, 46(5), p. 401-5.
[7] M.J. Abad, L.M. Bedoya, L. Apaza and P. Bermejo, “The Artemisia L. Genus: a review of bioactive essential oils. Molecules,” 2012, 17(3), p. 2542-66.
[8] M. Aziz , A. Karim , M. El Ouariachi , A. Bouyanzer , S. Amrani , H. Mekhfi , et al, “Relaxant Effect of Essential Oil of Artemisia herba-alba Asso. on Rodent Jejunum Contractions,” Sci Pharm, 2012, 80(2), p.457-467.
[9] M. Ballero, F. Poli, G. Sacchetti and M.C. Loi, “Ethnobotanical research on the territory of Fluminimaggiore (southwestern Sardinia), Fitoterapia, 2001, 72, p. 788–801.
[10] M. El Beyrouthy, N.A. Apostolides, M. Labaki, F. Cazier, S. Najm and A. Abou Kaïs, “Chemical composition of the essential oil of the Artemisia arborescens L. growing wild in lebanon,” LSJ, 2011, 12(1) p.71-78.
[11] G. Bachir Raho and M. Benali, “Antibacterial activity of the essential oils from the leaves of Eucalyptus globulus against Escherichia coli and Staphylococcus aureus,”Asian Pac J Trop Biomed, 2012, 2(9), p. 739–742.
[12] L.M. Lima, B. Babakhani, S.A.H. Boldaji, M. Asadi and R.M. Boldaji, “Essential oils composition and antibacterial activities of Eucalyptus camaldulensis Dehn,” Int. J. Med. Arom. Plants, 2013, 3(2), p. 214-219.
[13] E.S. Baykan, G. Reznicek, S.G. Senol, N.U. Karabay Yavasogul, S. Konyalioglu, A. Ulvi Zeybek, “ Antimicrobial and antioxidant properties of Artemisia L. species from western Anatolia,” Turk. J.Biol, 2012, 36, p.75–84.
[14] K. Younes, S. Merghache, N. Djabou, D. Merghache, A. Muselli, B. Tabti and J. Costa, “Chemical composition, antibacterial and antioxidant activities of a new essential oil chemotype of Algerian Artemisia arborescens L,” Afr. J. Pharm. Pharmacol, 2012, 6(42) , p. 2912-2921.
[15] M. Saddi , A. Sanna , F. Cottiglia , L. Chisu , L. Casu , L. Bonsignore , A. De Logu, “ Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells,” Ann Clin Microbiol Antimicrob, 2007, 6(10), 7p.
[16] A. Abderrahim, K. Belhamel, J.C. Chalchat and G. Figuérédo, “Chemical Composition of the Essential Oil from Artemisia arborescens L. Growing Wild in Algeria,” Rec. Nat. Prod, 2010, 4(1), p. 87-90.
[17] M. Militello , L. Settanni , A. Aleo , C. Mammina , G. Moschetti , G.M. Giammanco , et al, “Chemical composition and antibacterial potential of Artemisia arborescens L. essential oil,” Curr Microbiol, 2011, 62(4), p.1274-81.
[18] W. Chen, I. Vermaak and A.Viljoen, “Camphor—A Fumigant during the Black Death and a Coveted Fragrant Wood in Ancient Egypt and Babylon—A Review,” Molecules, 2013, 18, p.5434-5454.
[19] D. Tsiri, K. Graikou, L. Pobłocka-Olech, M. Krauze-Baranowska, C. Spyropoulos and I. Chinou, “Chemosystematic value of the essential oil composition of Thuja species cultivated in Poland-antimicrobial activity,” Molecules, 2009, 14(11), p. 4707-15.
[20] P. Owlia, L. Rasooli and H. Saderi, “Antistreptococcal and Antioxidant Activity of Essential Oil from Matricaria chamomilla L,” Res. J. Biol. Sci, 2007, 2 (2), p.155-160.
[21] A. Ben Arfa, S. Combes, L. Preziosi-Belloy , N. Gontard and P. Chalier, “Antimicrobial activity of carvacrol related to its chemical structure,” Lett Appl Microbiol, 2006, 43(2) , p.149-54.
[22] A. Santiesteban-López , E. Palou and A. López-Malo, “Susceptibility of food-borne bacteria to binary combinations of antimicrobials at selected a(w) and pH,” J Appl Microbiol, 2007, 102(2) , p. 486-97.
[23] S. Cosentino, C.I.G. Tuberoso, B. Pisano, M. Satta, V. Mascia, E. Arzedi, F. Palmas, “In-vitro antimicrobial activity and chemical composition of Sardinian thymus essential oils,” Lett Appl Microbiol, 1999, 29, p. 130–135.
[24] L.M. Leandro, F.S.Vargas, P.C.S. Barbosa, J.K.O. Neves, J.A. Silva and V.F. Veiga-Junior, “Chemistry and Biological Activities of Terpenoids from Copaiba (Copaifera spp.) Oleoresins,” Molecules, 2012, 17(4), p. 3866-3889.
[25] M. Mahboubi and N. Farzin, “Antimicrobial activity of Artemisia sieberi essential oil from central Iran,” Iran J Microbiol, 2009, 1(2), p. 43-48.
[26] P. Rattanachaikunsopon and P. Phumkhachorn, “Synergistic antimicrobial effect of nisin and p-cymene on Salmonella enterica serovar Typhi in vitro and on ready-to-eat food,” Biosci Biotechnol Biochem, 2010, 74(3), p. 520-4.
[27] N. Lattaoui and A. Tantaoui-Elaraki, “Individual and combined oils,” Rivista Italiana EPPOS, 1994,13, p.13–19.
[28] M.Marino, C. Bersani and G. Comi, “Impedance measurements to study the antimicrobial activity of essential oils from Lamiaceace and Compositae,” Int. J. Food Microbiol, 2001, 67, p. 187–195.
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  • APA Style

    Bachir Raho Ghalem. (2015). Effect of Essential Oils of Artemisia arborescens on Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Journal of Plant Sciences, 2(6-1), 1-4. https://doi.org/10.11648/j.jps.s.2014020601.11

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    ACS Style

    Bachir Raho Ghalem. Effect of Essential Oils of Artemisia arborescens on Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. J. Plant Sci. 2015, 2(6-1), 1-4. doi: 10.11648/j.jps.s.2014020601.11

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    AMA Style

    Bachir Raho Ghalem. Effect of Essential Oils of Artemisia arborescens on Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. J Plant Sci. 2015;2(6-1):1-4. doi: 10.11648/j.jps.s.2014020601.11

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  • @article{10.11648/j.jps.s.2014020601.11,
      author = {Bachir Raho Ghalem},
      title = {Effect of Essential Oils of Artemisia arborescens on Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa},
      journal = {Journal of Plant Sciences},
      volume = {2},
      number = {6-1},
      pages = {1-4},
      doi = {10.11648/j.jps.s.2014020601.11},
      url = {https://doi.org/10.11648/j.jps.s.2014020601.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.s.2014020601.11},
      abstract = {This study was carried out to investigate the potential use of Artemisia arborescens as a source of antimicrobial agents against pathogens. Essential oils of A. arborescens were collected by hydrodistillation. The antibacterial properties of A. arborescens essential oil was studied on the standard gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and Staphylococcus aureus (gram-positive bacteria), then agar disk diffusion, minimal inhibition concentration (MIC) and minimum bactericidal concentration (MBC) were detected. The results of agar disk diffusion tests showed the inhibition zones as follow: S. aureus 00-18 mm, E. coli 00-16 mm and 08-14 mm for P. aeruginosa. However, their antibacterial activities were lower than those of Gentamicin. The MIC for S. aureus and P. aeruginosa was between 33 and 66 mg/ml, and for Gram-negative bacteria of E. coli was between 66 and 132 mg/ml, while the MBC values of this oil against the tested bacterial strains were between 132 and 264 mg/ml.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Effect of Essential Oils of Artemisia arborescens on Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa
    AU  - Bachir Raho Ghalem
    Y1  - 2015/03/14
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    DO  - 10.11648/j.jps.s.2014020601.11
    T2  - Journal of Plant Sciences
    JF  - Journal of Plant Sciences
    JO  - Journal of Plant Sciences
    SP  - 1
    EP  - 4
    PB  - Science Publishing Group
    SN  - 2331-0731
    UR  - https://doi.org/10.11648/j.jps.s.2014020601.11
    AB  - This study was carried out to investigate the potential use of Artemisia arborescens as a source of antimicrobial agents against pathogens. Essential oils of A. arborescens were collected by hydrodistillation. The antibacterial properties of A. arborescens essential oil was studied on the standard gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa), and Staphylococcus aureus (gram-positive bacteria), then agar disk diffusion, minimal inhibition concentration (MIC) and minimum bactericidal concentration (MBC) were detected. The results of agar disk diffusion tests showed the inhibition zones as follow: S. aureus 00-18 mm, E. coli 00-16 mm and 08-14 mm for P. aeruginosa. However, their antibacterial activities were lower than those of Gentamicin. The MIC for S. aureus and P. aeruginosa was between 33 and 66 mg/ml, and for Gram-negative bacteria of E. coli was between 66 and 132 mg/ml, while the MBC values of this oil against the tested bacterial strains were between 132 and 264 mg/ml.
    VL  - 2
    IS  - 6-1
    ER  - 

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Author Information
  • Department of Biology, University of Mascara, Mascara, Algeria

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