This study focuses on the estimation and characterization of microorganisms isolated from wastewater and sludge in Awka metropolis, Nigeria. The samples were collected randomly from four (A, B, C and D) respective areas, analyzed microbiologically by homogenizing the samples under aseptic conditions to obtain a homogenous mixture, the isolation techniques used was pour plate methods as described by Cheesbrough, (2010); Joanne et al, (2011); APHA, (1989). The samples were diluted serially, inoculated on to MacConkey agar and Sabouraud Dextrose agar media each and microorganisms were isolated; wastewater had a range from 44 to 100 colonies and total of 284 (71 ± 24.30), CFU / ml (10-4) of 1.0 × 107 to 8.0 × 106 with total count of 19.4 (4.85 ± 2.96) × 106, while sludge was ranges from 60 to 120 colonies with a total of 358 (89.5 ± 29), CFU / ml (10-4) ranged from 1.0 × 107 to 7.0 × 106 with total count of 15.2 (3.8 ± 3.1) × 106. The colony and the morphology of the isolates were recorded, the bacterial isolates were characterized by motility tests, Gram staining and identified by biochemical tests. The fungal isolates were microscopically examined using a wet mount procedure. From the results obtained 8 bacterial and 7 fungal isolates were identified from both the wastewater and the sludge; Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Micrococcus leteus, Enterococcus faecalis, Serratia mercescens and Yersinia enterocolitica. Acrophialophora fusispora, Epicoccum Purpurascens, Rhizopus microsporus, Exophiala spinifera, Cladosporium cladosporiodes, Aspergillus niger and Phaeoacremonium parasiticum. Results also showed that staphylococcus aureus have the highest percentage of occurrence in wastewater, while in sludge Micrococcus species have the highest percentage of occurrence.
| Published in |
International Journal of Environmental Protection and Policy (Volume 5, Issue 6-1)
This article belongs to the Special Issue The Role of SLT: Environmental Impact Assessment And Statement Concept |
| DOI | 10.11648/j.ijepp.s.2017050601.14 |
| Page(s) | 23-32 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2017. Published by Science Publishing Group |
Biochemical Test, Culture Media, Domestic, Industrial, Microorganism Morphological, Sludge, Wastewater
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APA Style
Bassey Effiong Edet, Gwana Adamu Mohammed, Buhari Bagudu Yawuri, Alhaji Bashir Mohammed, Abubakar Musa, et al. (2017). Microbial Estimation and Characterization of Wastewater and Sludge in Awka Metropolis, Nigeria. International Journal of Environmental Protection and Policy, 5(6-1), 23-32. https://doi.org/10.11648/j.ijepp.s.2017050601.14
ACS Style
Bassey Effiong Edet; Gwana Adamu Mohammed; Buhari Bagudu Yawuri; Alhaji Bashir Mohammed; Abubakar Musa, et al. Microbial Estimation and Characterization of Wastewater and Sludge in Awka Metropolis, Nigeria. Int. J. Environ. Prot. Policy 2017, 5(6-1), 23-32. doi: 10.11648/j.ijepp.s.2017050601.14
AMA Style
Bassey Effiong Edet, Gwana Adamu Mohammed, Buhari Bagudu Yawuri, Alhaji Bashir Mohammed, Abubakar Musa, et al. Microbial Estimation and Characterization of Wastewater and Sludge in Awka Metropolis, Nigeria. Int J Environ Prot Policy. 2017;5(6-1):23-32. doi: 10.11648/j.ijepp.s.2017050601.14
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Download@article{10.11648/j.ijepp.s.2017050601.14,
author = {Bassey Effiong Edet and Gwana Adamu Mohammed and Buhari Bagudu Yawuri and Alhaji Bashir Mohammed and Abubakar Musa and Abba Mala Galtimari and Hauwa Lawan Badawi and Mustapha Ahmadu and Fanna Ali Garra},
title = {Microbial Estimation and Characterization of Wastewater and Sludge in Awka Metropolis, Nigeria},
journal = {International Journal of Environmental Protection and Policy},
volume = {5},
number = {6-1},
pages = {23-32},
doi = {10.11648/j.ijepp.s.2017050601.14},
url = {https://doi.org/10.11648/j.ijepp.s.2017050601.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijepp.s.2017050601.14},
abstract = {This study focuses on the estimation and characterization of microorganisms isolated from wastewater and sludge in Awka metropolis, Nigeria. The samples were collected randomly from four (A, B, C and D) respective areas, analyzed microbiologically by homogenizing the samples under aseptic conditions to obtain a homogenous mixture, the isolation techniques used was pour plate methods as described by Cheesbrough, (2010); Joanne et al, (2011); APHA, (1989). The samples were diluted serially, inoculated on to MacConkey agar and Sabouraud Dextrose agar media each and microorganisms were isolated; wastewater had a range from 44 to 100 colonies and total of 284 (71 ± 24.30), CFU / ml (10-4) of 1.0 × 107 to 8.0 × 106 with total count of 19.4 (4.85 ± 2.96) × 106, while sludge was ranges from 60 to 120 colonies with a total of 358 (89.5 ± 29), CFU / ml (10-4) ranged from 1.0 × 107 to 7.0 × 106 with total count of 15.2 (3.8 ± 3.1) × 106. The colony and the morphology of the isolates were recorded, the bacterial isolates were characterized by motility tests, Gram staining and identified by biochemical tests. The fungal isolates were microscopically examined using a wet mount procedure. From the results obtained 8 bacterial and 7 fungal isolates were identified from both the wastewater and the sludge; Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Micrococcus leteus, Enterococcus faecalis, Serratia mercescens and Yersinia enterocolitica. Acrophialophora fusispora, Epicoccum Purpurascens, Rhizopus microsporus, Exophiala spinifera, Cladosporium cladosporiodes, Aspergillus niger and Phaeoacremonium parasiticum. Results also showed that staphylococcus aureus have the highest percentage of occurrence in wastewater, while in sludge Micrococcus species have the highest percentage of occurrence.},
year = {2017}
}
TY - JOUR T1 - Microbial Estimation and Characterization of Wastewater and Sludge in Awka Metropolis, Nigeria AU - Bassey Effiong Edet AU - Gwana Adamu Mohammed AU - Buhari Bagudu Yawuri AU - Alhaji Bashir Mohammed AU - Abubakar Musa AU - Abba Mala Galtimari AU - Hauwa Lawan Badawi AU - Mustapha Ahmadu AU - Fanna Ali Garra Y1 - 2017/08/21 PY - 2017 N1 - https://doi.org/10.11648/j.ijepp.s.2017050601.14 DO - 10.11648/j.ijepp.s.2017050601.14 T2 - International Journal of Environmental Protection and Policy JF - International Journal of Environmental Protection and Policy JO - International Journal of Environmental Protection and Policy SP - 23 EP - 32 PB - Science Publishing Group SN - 2330-7536 UR - https://doi.org/10.11648/j.ijepp.s.2017050601.14 AB - This study focuses on the estimation and characterization of microorganisms isolated from wastewater and sludge in Awka metropolis, Nigeria. The samples were collected randomly from four (A, B, C and D) respective areas, analyzed microbiologically by homogenizing the samples under aseptic conditions to obtain a homogenous mixture, the isolation techniques used was pour plate methods as described by Cheesbrough, (2010); Joanne et al, (2011); APHA, (1989). The samples were diluted serially, inoculated on to MacConkey agar and Sabouraud Dextrose agar media each and microorganisms were isolated; wastewater had a range from 44 to 100 colonies and total of 284 (71 ± 24.30), CFU / ml (10-4) of 1.0 × 107 to 8.0 × 106 with total count of 19.4 (4.85 ± 2.96) × 106, while sludge was ranges from 60 to 120 colonies with a total of 358 (89.5 ± 29), CFU / ml (10-4) ranged from 1.0 × 107 to 7.0 × 106 with total count of 15.2 (3.8 ± 3.1) × 106. The colony and the morphology of the isolates were recorded, the bacterial isolates were characterized by motility tests, Gram staining and identified by biochemical tests. The fungal isolates were microscopically examined using a wet mount procedure. From the results obtained 8 bacterial and 7 fungal isolates were identified from both the wastewater and the sludge; Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Micrococcus leteus, Enterococcus faecalis, Serratia mercescens and Yersinia enterocolitica. Acrophialophora fusispora, Epicoccum Purpurascens, Rhizopus microsporus, Exophiala spinifera, Cladosporium cladosporiodes, Aspergillus niger and Phaeoacremonium parasiticum. Results also showed that staphylococcus aureus have the highest percentage of occurrence in wastewater, while in sludge Micrococcus species have the highest percentage of occurrence. VL - 5 IS - 6-1 ER -
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